{"product_id":"tfdp3-antibody-transcription-factor-dp-family-member-3-bha17135365","title":"TFDP3 Antibody \/ Transcription factor Dp family member 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTFDP3 Antibody \/ Transcription factor Dp family member 3 is a anti-TFDP3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA with listed reactivity in Human. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TFDP3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTFDP3 antibody recognizes Transcription factor Dp family member 3, a unique atypical DP protein that modulates E2F-dependent transcription and cell cycle regulation. Unlike canonical DP proteins (TFDP1 and TFDP2), TFDP3 acts as a transcriptional repressor rather than an activator. It binds to E2F transcription factors but lacks the transactivation domain necessary for promoting gene expression, thereby acting as a competitive inhibitor. The TFDP3 antibody is an essential tool for studying cell proliferation, differentiation, apoptosis, and oncogenic transformation regulated through the E2F\/DP pathway.\u003cbr\u003e\u003cbr\u003eTFDP3 is encoded by the TFDP3 gene located on human chromosome Xq26.2. The protein consists of a conserved DNA-binding domain and a dimerization region that facilitates complex formation with E2F1. This heterodimerization blocks the transcriptional activation of E2F target genes involved in DNA synthesis and cell cycle progression. As a result, TFDP3 contributes to cell cycle arrest and resistance to apoptosis, especially under stress or DNA damage conditions.\u003cbr\u003e\u003cbr\u003eThe TFDP3 antibody is used to detect the 45-47 kDa nuclear protein in various cell lines and tissues. Immunofluorescence reveals nuclear localization consistent with its function as a transcriptional regulator. TFDP3 is predominantly expressed in testis, certain stem cells, and a subset of cancer tissues. Its overexpression has been reported in hepatocellular carcinoma, lung adenocarcinoma, and breast cancer, where it promotes survival by suppressing E2F1-induced apoptosis. Mechanistically, TFDP3 interferes with E2F1's ability to induce p53-independent cell death, thereby facilitating oncogenic proliferation.\u003cbr\u003e\u003cbr\u003eBeyond cell cycle regulation, TFDP3 participates in differentiation processes and developmental gene expression control. It is also implicated in DNA repair regulation and stress-induced transcriptional reprogramming. Studies have shown that silencing TFDP3 enhances the cytotoxic effects of chemotherapeutic agents, making it a potential therapeutic target.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TFDP3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TFDP3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TFDP3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TFDP3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TFDP3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289315693,"sku":"FY12462","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_af5521fd-f268-43f7-abbb-9c2e58ec95dc.jpg?v=1782237005","url":"https:\/\/www.ebiohippo.com\/products\/tfdp3-antibody-transcription-factor-dp-family-member-3-bha17135365","provider":"BioHippo","version":"1.0","type":"link"}