{"product_id":"tfec-antibody-transcription-factor-ec-bha17135455","title":"TFEC Antibody \/ Transcription factor EC","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTFEC Antibody \/ Transcription factor EC is a anti-TFEC Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TFEC\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTFEC antibody detects Transcription factor EC, a basic helix-loop-helix leucine zipper (bHLH-LZ) transcription factor that regulates gene expression in macrophages, osteoclasts, and melanocytes. TFEC belongs to the MiT\/TFE family, which includes MITF, TFE3, and TFEB, all of which coordinate transcriptional responses to nutrient availability, lysosomal function, and immune signaling. The TFEC antibody is widely used in transcriptional regulation, macrophage differentiation, and cancer research.\u003cbr\u003e\u003cbr\u003eTFEC is encoded by the TFEC gene located on human chromosome 7q31.2. The protein is approximately 38 kilodaltons and contains a conserved DNA-binding bHLH-LZ domain that mediates binding to E-box sequences in gene promoters. TFEC forms homodimers or heterodimers with other MiT family members, allowing versatile regulation of target gene networks. It localizes to the nucleus and is activated by phosphorylation under stress or inflammatory conditions.\u003cbr\u003e\u003cbr\u003eThe TFEC antibody detects a 38 kilodalton protein by western blot and demonstrates nuclear staining in macrophages and osteoclast precursors. TFEC regulates transcription of lysosomal and autophagy-related genes, participating in cellular adaptation to nutrient deprivation. It also modulates inflammatory gene expression through cooperation with PU.1 and other macrophage-specific transcription factors, promoting cytokine and chemokine production.\u003cbr\u003e\u003cbr\u003eIn melanocytes, TFEC contributes to pigment cell differentiation and interacts with MITF to balance melanogenic gene expression. Dysregulation of TFEC has been implicated in cancer, where fusion genes involving TFEC drive aberrant transcriptional programs. Altered expression is also associated with inflammatory and metabolic disorders due to disrupted lysosomal signaling and autophagic control.\u003cbr\u003e\u003cbr\u003eTFEC integrates environmental and metabolic signals to maintain homeostasis in immune and pigment cells.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TFEC - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TFEC - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TFEC - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TFEC - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TFEC+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047292789101,"sku":"FY12552","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f27ff2d1-e079-4e22-a039-4213119953ef.jpg?v=1782237027","url":"https:\/\/www.ebiohippo.com\/products\/tfec-antibody-transcription-factor-ec-bha17135455","provider":"BioHippo","version":"1.0","type":"link"}