{"product_id":"tgif2-antibody-tg-interacting-factor-2-bha17136067","title":"TGIF2 Antibody \/ TG-interacting factor 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTGIF2 Antibody \/ TG-interacting factor 2 is a anti-TGIF2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human. Reported localization: Nucleus, Centrosome.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TGIF2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, IP, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTGIF2 antibody detects TG-interacting factor 2, a homeodomain transcriptional repressor that modulates TGF-beta signaling and developmental gene expression. The UniProt recommended name is TG-interacting factor 2 (TGIF2). This nuclear protein belongs to the TALE (three-amino acid loop extension) class of homeobox proteins and functions as a DNA-binding corepressor that inhibits Smad-mediated transcription.\u003cbr\u003e\u003cbr\u003eFunctionally, TGIF2 antibody identifies a 237-amino-acid protein localized in the nucleus, where it binds DNA at consensus homeobox sites and interacts with transcriptional repressors such as CtBP and HDACs. TGIF2 represses TGF-beta target genes by blocking Smad binding to promoters, fine-tuning cellular differentiation, proliferation, and morphogenesis during embryonic development.\u003cbr\u003e\u003cbr\u003eThe TGIF2 gene is located on chromosome 20q11.2 and is expressed in embryonic tissues and adult organs including brain, kidney, and testis. TGIF2 acts as a developmental regulator controlling anterior-posterior patterning and organ formation through transcriptional repression mechanisms.\u003cbr\u003e\u003cbr\u003ePathologically, TGIF2 overexpression has been observed in several cancers, including ovarian and gastric tumors, where it promotes cell proliferation and epithelial-mesenchymal transition. Gene duplications or deletions involving TGIF2 have been associated with holoprosencephaly and craniofacial malformations. Research using TGIF2 antibody supports studies in transcriptional repression, developmental biology, and oncogenesis.\u003cbr\u003e\u003cbr\u003eTGIF2 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect homeodomain transcription factors. \u003cbr\u003e\u003cbr\u003eStructurally, TG-interacting factor 2 contains a homeobox DNA-binding domain and a CtBP-binding motif that mediate its repressor activity. This antibody aids in analyzing TGIF2's role in gene regulation, morphogenesis, and signaling cross-talk.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TGIF2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TGIF2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TGIF2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TGIF2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TGIF2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314153837,"sku":"FY13165","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8e3ccee9-4222-4efc-ac09-92381b817bbb.jpg?v=1782237114","url":"https:\/\/www.ebiohippo.com\/products\/tgif2-antibody-tg-interacting-factor-2-bha17136067","provider":"BioHippo","version":"1.0","type":"link"}