{"product_id":"tgm7-antibody-transglutaminase-7-bha17136250","title":"TGM7 Antibody \/ Transglutaminase 7","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTGM7 Antibody \/ Transglutaminase 7 is a anti-TGM7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TGM7\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTGM7 antibody detects Transglutaminase 7, a cytoplasmic and membrane-associated enzyme encoded by the TGM7 gene on chromosome 15q15.2. TGM7 belongs to the transglutaminase enzyme family and catalyzes calcium-dependent protein crosslinking by forming ?-(gamma-glutamyl)lysine bonds between proteins. This post-translational modification stabilizes protein assemblies and contributes to epithelial differentiation, wound healing, and barrier function. TGM7 is expressed in skin, gastrointestinal epithelium, and reproductive tissues, with developmental expression during keratinocyte maturation and spermatogenesis.\u003cbr\u003e\u003cbr\u003eFunctionally, TGM7 participates in the formation of insoluble protein structures within epithelial cells, supporting tissue integrity under mechanical and environmental stress. It crosslinks structural proteins such as keratins, involucrin, and loricrin, enhancing the strength of epithelial layers. Co-localization studies show TGM7 associating with the plasma membrane and cytoskeletal components during terminal differentiation of keratinocytes. The enzyme also contributes to barrier maintenance in the gastrointestinal tract and reproductive system.\u003cbr\u003e\u003cbr\u003eStructurally, TGM7 shares conserved catalytic residues with other transglutaminases, including the catalytic triad of cysteine, histidine, and aspartate. It contains a core transamidase domain flanked by beta-sandwich and beta-barrel domains that facilitate substrate recognition and enzymatic activity. TGM7 is part of the transglutaminase enzyme family, which includes TGM1-6 and Factor XIIIa, all of which catalyze similar crosslinking reactions. Calcium binding activates TGM7, promoting conformational changes that expose the catalytic site for substrate processing.\u003cbr\u003e\u003cbr\u003eTGM7 plays roles beyond structural reinforcement, including regulation of cell adhesion, apoptosis, and immune responses. It has been shown to modulate integrin signaling and extracellular matrix interactions. Dysregulation of TGM7 expression is linked to skin disorders, inflammatory bowel disease, and cancer. Overexpression has been observed in colorectal and pancreatic tumors, where TGM7 may promote cell migration and invasion through cytoskeletal remodeling. Pathway involvement includes keratinocyte differentiation, cell adhesion, and calcium signaling.\u003cbr\u003e\u003cbr\u003eTissue-specific studies indicate that TGM7 expression is high in differentiated epithelial cells and germ cells, reflecting its contribution to terminal differentiation and reproductive tissue function. During embryonic development, it supports epithelial morphogenesis and barrier establishment. Its enzymatic crosslinking activity provides stability to tissues exposed to environmental stressors such as mechanical abrasion and chemical insult.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using TGM7 antibody shows cytoplasmic and membrane localization in epithelial tissues and testis. The TGM7 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TGM7 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TGM7 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TGM7 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TGM7 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TGM7+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047322902893,"sku":"FY13348","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_50cad63b-821e-4fd5-98b9-1fd13efc33e5.jpg?v=1782237105","url":"https:\/\/www.ebiohippo.com\/products\/tgm7-antibody-transglutaminase-7-bha17136250","provider":"BioHippo","version":"1.0","type":"link"}