{"product_id":"them6-antibody-thioesterase-superfamily-member-6-bha17135929","title":"THEM6 Antibody \/ Thioesterase superfamily member 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTHEM6 Antibody \/ Thioesterase superfamily member 6 is a anti-THEM6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e THEM6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTHEM6 antibody detects Acyl-CoA thioesterase THEM6, a lipid-metabolizing enzyme that hydrolyzes fatty acyl-CoA esters to free fatty acids and CoA, regulating lipid signaling and energy homeostasis. The UniProt recommended name is Acyl-CoA thioesterase THEM6 (THEM6). This enzyme belongs to the thioesterase superfamily, which plays essential roles in lipid metabolism, peroxisomal function, and cellular stress response.\u003cbr\u003e\u003cbr\u003eFunctionally, THEM6 antibody identifies a 247-amino-acid protein localized primarily in the endoplasmic reticulum (ER). THEM6 catalyzes the hydrolysis of long-chain acyl-CoA molecules, controlling intracellular levels of activated fatty acids and maintaining the balance between lipid synthesis and degradation. By regulating acyl-CoA and free fatty acid pools, THEM6 influences energy metabolism, membrane composition, and lipid signaling cascades.\u003cbr\u003e\u003cbr\u003eThe THEM6 gene is located on chromosome 1p36.33 and encodes an enzyme expressed in metabolically active tissues, including liver, adipose tissue, and skeletal muscle. THEM6 contains a hotdog-fold thioesterase domain characteristic of enzymes that process acyl-CoA intermediates. Its activity is critical for preventing the accumulation of toxic acyl-CoA derivatives that can impair mitochondrial function and oxidative metabolism. THEM6 also participates in lipid remodeling pathways that maintain ER membrane homeostasis.\u003cbr\u003e\u003cbr\u003eIn cellular metabolism, THEM6 acts as a regulator of lipid droplet dynamics and fatty acid oxidation. It modulates the availability of CoA for beta-oxidation and phospholipid biosynthesis, indirectly influencing mitochondrial energy production. Dysregulation of THEM6 expression has been linked to metabolic disorders, including fatty liver disease and obesity. Emerging evidence suggests a role for THEM6 in cancer cell metabolism, where enhanced lipid turnover supports rapid proliferation and membrane biogenesis.\u003cbr\u003e\u003cbr\u003eTHEM6 antibody is widely used in lipid metabolism, enzymology, and metabolic disease research. It is suitable for western blotting, immunofluorescence, and subcellular localization studies to detect THEM6 expression in cellular and tissue samples. This antibody supports studies of acyl-CoA metabolism, lipid homeostasis, and ER stress responses. In metabolic and cancer research, THEM6 detection provides insights into how cells reprogram lipid utilization under stress or nutrient limitation.\u003cbr\u003e\u003cbr\u003eStructurally, THEM6 contains a conserved thioesterase catalytic triad composed of serine, histidine, and aspartate residues, mediating acyl-CoA hydrolysis. Its ER localization is maintained through a C-terminal retention signal, and its expression may be regulated by nutrient and hormonal cues.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=THEM6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=THEM6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=THEM6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/THEM6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=THEM6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310319981,"sku":"FY13027","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d6ad6735-3d4e-4850-842a-76d41da75a43.jpg?v=1782237068","url":"https:\/\/www.ebiohippo.com\/products\/them6-antibody-thioesterase-superfamily-member-6-bha17135929","provider":"BioHippo","version":"1.0","type":"link"}