{"product_id":"thiol-fluorometric-assay-kit-bht19600014","title":"Thiol Fluorometric Assay Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eMetabolite Assay Kit\u003c\/span\u003e\u003ch2\u003eAbout the Analyte\u003c\/h2\u003e\n\u003cp\u003eFree thiols, overwhelmingly contributed by glutathione and protein cysteines, buffer the cell against oxidative insult, and their depletion is one of the earliest measurable signs of redox imbalance.\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThe Thiol Fluorometric Assay Kit applies a fluorometric format to this measurement, read at Ex\/Em = 400\/465 nm. Hands-on and incubation time is 10 minutes.\u003c\/p\u003e\n\u003cp\u003eThiols are extremely efficient antioxidants that can protect cellular lipids, proteins, and nucleic acids against peroxidative damage due to their strong reductive capacity and their ability to react with free radicals.\u003c\/p\u003e\n\u003cp\u003eMeasuring total free thiol captures both glutathione and protein cysteines, giving a broader picture of redox buffering than GSH alone.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eFluorometric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Wavelength\u003c\/th\u003e\n\u003ctd\u003eEx\/Em = 400\/465 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e0.3-20 µM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Time\u003c\/th\u003e\n\u003ctd\u003e10 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Types\u003c\/th\u003e\n\u003ctd\u003ecells, tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e100 tests\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at -20°C. Shelf life: 1 year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMetabolic Assay\u003c\/li\u003e\n\u003cli\u003eROS Detection\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C. Shelf life: 1 year.\u003c\/p\u003e\n\u003ch2\u003eSafety\u003c\/h2\u003e\n\u003cp\u003eFor research use only. Not for use in diagnostic or therapeutic procedures. Handle in accordance with good laboratory practice; consult the SDS before use.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"100 tests","offer_id":53652622606701,"sku":"TBS2026","price":468.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TBS2026.jpg?v=1785879139","url":"https:\/\/www.ebiohippo.com\/products\/thiol-fluorometric-assay-kit-bht19600014","provider":"BioHippo","version":"1.0","type":"link"}