{"product_id":"thrap3-antibody-trap150-thyroid-hormone-receptor-associated-protein-3-bha17136047","title":"THRAP3 Antibody \/ TRAP150 \/ Thyroid hormone receptor-associated protein 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTHRAP3 Antibody \/ TRAP150 \/ Thyroid hormone receptor-associated protein 3 is a anti-THRAP3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e THRAP3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTHRAP3 antibody detects Thyroid hormone receptor-associated protein 3, an RNA-binding and transcriptional coactivator involved in mRNA splicing, export, and DNA damage response. The UniProt recommended name is Thyroid hormone receptor-associated protein 3 (THRAP3). This multifunctional nuclear protein integrates transcriptional and post-transcriptional regulation, linking chromatin signaling to RNA metabolism.\u003cbr\u003e\u003cbr\u003eFunctionally, THRAP3 antibody identifies a 955-amino-acid protein that associates with transcriptional coactivator complexes, including TRAP\/SMCC\/Med complex components, and with splicing regulators. THRAP3 modulates alternative splicing of pre-mRNAs and facilitates the export of mature transcripts to the cytoplasm. It also interacts with BRCA1 and phosphorylated proteins in response to DNA damage, influencing genome stability.\u003cbr\u003e\u003cbr\u003eThe THRAP3 gene is located on chromosome 1p36.11 and is ubiquitously expressed in proliferating tissues. Through its binding to RNA and chromatin-associated factors, THRAP3 functions as a bridge between transcriptional activation and mRNA maturation, coordinating gene expression output.\u003cbr\u003e\u003cbr\u003ePathologically, THRAP3 dysfunction has been linked to cancer and metabolic diseases. Aberrant splicing or mutations affecting THRAP3 lead to impaired mRNA processing and genomic instability. Research using THRAP3 antibody supports studies in transcriptional control, splicing regulation, and DNA repair signaling.\u003cbr\u003e\u003cbr\u003eTHRAP3 antibody is validated for western blotting, immunoprecipitation, and immunofluorescence to detect RNA-binding coactivator proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Thyroid hormone receptor-associated protein 3 contains low-complexity domains and serine\/arginine-rich regions that mediate protein-RNA and protein-protein interactions. This antibody enables exploration of THRAP3's molecular role in coordinating transcriptional activation with RNA processing.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=THRAP3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=THRAP3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=THRAP3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/THRAP3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=THRAP3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047313564013,"sku":"FY13145","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_60fca080-3415-47bf-87bd-d2607f24308f.jpg?v=1782237099","url":"https:\/\/www.ebiohippo.com\/products\/thrap3-antibody-trap150-thyroid-hormone-receptor-associated-protein-3-bha17136047","provider":"BioHippo","version":"1.0","type":"link"}