{"product_id":"tigar-antibody-tp53-induced-glycolysis-and-apoptosis-regulator-bha17135500","title":"TIGAR Antibody \/ TP53-induced glycolysis and apoptosis regulator","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTIGAR Antibody \/ TP53-induced glycolysis and apoptosis regulator is a anti-TIGAR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA with listed reactivity in Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TIGAR\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTIGAR antibody detects TP53-induced glycolysis and apoptosis regulator, a fructose-2,6-bisphosphatase-like enzyme that links p53 signaling to metabolic control and cell survival. TIGAR modulates glycolytic flux and antioxidant defense, protecting cells from oxidative stress. The TIGAR antibody is widely used in cancer metabolism, apoptosis, and redox biology research to study metabolic adaptation, p53 regulation, and energy homeostasis.\u003cbr\u003e\u003cbr\u003eTIGAR is encoded by the TIGAR gene located on human chromosome 12p13.31. The protein is approximately 270 amino acids in length and structurally resembles the bisphosphatase domain of phosphofructokinase-2\/fructose-2,6-bisphosphatase (PFKFB) enzymes. However, TIGAR lacks kinase activity and instead functions primarily as a fructose-2,6-bisphosphatase, reducing levels of fructose-2,6-bisphosphate and thereby suppressing glycolysis while promoting the pentose phosphate pathway (PPP).\u003cbr\u003e\u003cbr\u003eThe TIGAR antibody detects a 30 kilodalton band by western blot and shows cytoplasmic and nuclear localization under immunofluorescence. Through its enzymatic activity, TIGAR decreases glycolytic intermediates and redirects glucose metabolism toward the PPP, enhancing production of NADPH and glutathione for antioxidant defense. This metabolic shift protects cells against oxidative stress and apoptosis, particularly under conditions of DNA damage or metabolic stress.\u003cbr\u003e\u003cbr\u003eRegulated directly by p53, TIGAR expression serves as a metabolic checkpoint balancing energy generation and redox homeostasis. In cancer, overexpression of TIGAR supports tumor cell survival by reducing reactive oxygen species (ROS) and maintaining mitochondrial integrity. Conversely, TIGAR inhibition sensitizes cells to oxidative damage and enhances the efficacy of chemotherapeutic agents.\u003cbr\u003e\u003cbr\u003eBeyond cancer metabolism, TIGAR plays roles in neuroprotection, cardiac remodeling, and ischemic tolerance. In neurons, TIGAR preserves mitochondrial function and reduces cell death after hypoxic injury. In cardiac tissue, TIGAR activation supports energy recovery following ischemia. These observations establish TIGAR as a critical regulator of metabolic plasticity and stress resilience.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TIGAR - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TIGAR - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TIGAR - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TIGAR - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TIGAR+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047293673837,"sku":"FY12597","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5e4cf7d1-06c4-4df2-b786-94d9589749a9.jpg?v=1782237023","url":"https:\/\/www.ebiohippo.com\/products\/tigar-antibody-tp53-induced-glycolysis-and-apoptosis-regulator-bha17135500","provider":"BioHippo","version":"1.0","type":"link"}