{"product_id":"tigar-antibody-tp53-induced-glycolysis-and-apoptosis-regulator-bha17135679","title":"TIGAR Antibody \/ TP53-induced glycolysis and apoptosis regulator","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTIGAR Antibody \/ TP53-induced glycolysis and apoptosis regulator is a anti-TIGAR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Mitochondria, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TIGAR\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTIGAR antibody detects TP53-induced glycolysis and apoptosis regulator, a key metabolic enzyme that modulates glycolysis, antioxidant defense, and cell survival. Encoded by the TIGAR gene on chromosome 12p13.3, this protein was identified as a transcriptional target of the tumor suppressor p53. TIGAR functions as a fructose-2,6-bisphosphatase, reducing levels of fructose-2,6-bisphosphate in the cytoplasm and thereby inhibiting glycolysis while promoting the pentose phosphate pathway. This metabolic shift enhances NADPH production, which supports antioxidant defense and protects cells against reactive oxygen species (ROS).\u003cbr\u003e\u003cbr\u003eTIGAR contains a bisphosphatase domain homologous to that of the PFKFB family of enzymes but functions independently to regulate metabolic flux. By lowering intracellular fructose-2,6-bisphosphate, it decreases glycolytic activity and reduces lactate production while increasing glucose utilization for nucleotide synthesis and redox balance. This activity allows TIGAR to protect cells from oxidative stress and apoptosis under p53-mediated stress responses. It also contributes to autophagy regulation by balancing energy production and redox homeostasis.\u003cbr\u003e\u003cbr\u003eThe TIGAR antibody is used in metabolism, cancer, and redox biology research to investigate metabolic reprogramming and p53-dependent signaling. Western blot analysis typically identifies a 30 kilodalton band corresponding to TIGAR, while immunofluorescence shows cytoplasmic and perinuclear staining in metabolically active cells. Because TIGAR regulates the balance between glycolysis and antioxidant pathways, this antibody is a valuable tool for studying stress responses, metabolic adaptation, and tumor metabolism.\u003cbr\u003e\u003cbr\u003eIn cancer, TIGAR overexpression can support tumor cell survival by reducing oxidative stress, contributing to chemoresistance and metabolic flexibility. Conversely, TIGAR deficiency enhances ROS accumulation and apoptosis, sensitizing cells to stress.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TIGAR - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TIGAR - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TIGAR - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TIGAR - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TIGAR+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299342701,"sku":"FY12776","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2a2deaad-1223-423d-91cb-9d160606bae7.jpg?v=1782237037","url":"https:\/\/www.ebiohippo.com\/products\/tigar-antibody-tp53-induced-glycolysis-and-apoptosis-regulator-bha17135679","provider":"BioHippo","version":"1.0","type":"link"}