{"product_id":"tim3-nfat-reporter-lentivirus-bhv19400275","title":"TIM3\/NFAT Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eTIM-3 (T cell immunoglobulin and mucin domain-containing protein 3, encoded by HAVCR2) is a co-inhibitory checkpoint receptor expressed on exhausted T cells, regulatory T cells, NK cells, and myeloid cells. Engagement by ligands such as galectin-9, phosphatidylserine, CEACAM1, and HMGB1 dampens T cell receptor signaling and effector function, contributing to immune tolerance and T cell exhaustion in chronic infection and cancer. Because TIM-3 engagement suppresses NFAT-dependent transcription downstream of the T cell receptor, NFAT-driven reporters provide a quantitative readout of TIM-3 signaling, and TIM-3 is an actively pursued checkpoint target in immuno-oncology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe TIM3\/NFAT Reporter Lentivirus is a two-vial immunotherapy reporter system for studying TIM-3 checkpoint signaling. One vial constitutively expresses the human TIM-3 receptor with an antibiotic selection marker, while the other carries tandem NFAT response elements driving a dual reporter combining secreted Gaussia luciferase (GLuc) and a fluorescent protein (GFP or RFP). Sequential transduction and selection generate a dual-stable effector cell line that responds quantitatively to receptor engagement. Secreted GLuc accumulates in conditioned media for kinetic, lysis-free sampling, while the fluorescent reporter enables microscopy and flow cytometry. Applications include evaluating immunotherapies targeting T cell exhaustion and characterizing TIM-3-mediated immune regulation. Supplied as high-titer, VSV-G-pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human TIM3 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251625058669,"sku":"TRV-0027-6S","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Vector-Layout-1024x261_571e2f7d-a5e3-431f-bb07-85ffcc0fd680.jpg?v=1782157779","url":"https:\/\/www.ebiohippo.com\/products\/tim3-nfat-reporter-lentivirus-bhv19400275","provider":"BioHippo","version":"1.0","type":"link"}