{"product_id":"tim50-antibody-translocase-of-inner-mitochondrial-membrane-50-bha17136028","title":"TIM50 Antibody \/ Translocase of inner mitochondrial membrane 50","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTIM50 Antibody \/ Translocase of inner mitochondrial membrane 50 is a anti-TIM50 Rabbit antibody Recombinant Rabbit Monoclonal clone 32T72 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TIM50\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32T72, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTIM50 antibody detects Translocase of inner mitochondrial membrane 50, encoded by the TIMM50 gene. Translocase of inner mitochondrial membrane 50, commonly called TIM50, is an essential component of the TIM23 complex that mediates the import of nuclear encoded mitochondrial proteins. The majority of mitochondrial proteins are encoded in the nucleus, synthesized in the cytoplasm, and imported across the mitochondrial membranes through specialized translocases. TIM50 antibody provides researchers with a critical reagent to study protein import, mitochondrial biogenesis, and energy metabolism.\u003cbr\u003e\u003cbr\u003eAs a receptor subunit of the TIM23 complex, TIM50 binds to presequences on precursor proteins as they enter the intermembrane space. Research using TIM50 antibody has shown that this interaction is required for recognition and transfer of proteins from the TOM complex in the outer membrane to the TIM23 channel in the inner membrane. TIM50 also acts as a gatekeeper, regulating the opening and closing of the translocation channel in response to membrane potential and precursor signals. This makes TIM50 indispensable for mitochondrial protein import.\u003cbr\u003e\u003cbr\u003eDysfunction of TIMM50 is linked to mitochondrial disease. Mutations in the TIMM50 gene cause combined oxidative phosphorylation deficiency, characterized by developmental delay, seizures, and lactic acidosis. Studies with TIM50 antibody have confirmed that mutations reduce protein import efficiency and disrupt mitochondrial homeostasis. Because proper protein import is essential for assembly of respiratory chain complexes, TIM50 deficiency leads to impaired energy metabolism and neuromuscular symptoms.\u003cbr\u003e\u003cbr\u003eBeyond rare genetic syndromes, TIM50 has been implicated in cancer and neurodegeneration. Research with TIM50 antibody has demonstrated altered expression in tumors, where mitochondrial remodeling supports rapid growth. In neurons, reduced TIM50 activity contributes to mitochondrial fragmentation and synaptic dysfunction. These findings highlight its broader significance beyond classical mitochondrial import pathways.\u003cbr\u003e\u003cbr\u003eTIM50 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects TIM50 expression in mitochondria rich tissues, immunohistochemistry localizes it to muscle and brain, and immunofluorescence confirms inner membrane localization with colocalization to mitochondrial markers. These applications make TIM50 antibody essential for studying mitochondrial protein transport.\u003cbr\u003e\u003cbr\u003eBy supplying validated TIM50 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TIM50 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TIM50 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TIM50 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TIM50 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TIM50+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047313072493,"sku":"FY13126","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_074af0f4-0cc9-43ea-bedb-b8c009324d77.jpg?v=1772019399","url":"https:\/\/www.ebiohippo.com\/products\/tim50-antibody-translocase-of-inner-mitochondrial-membrane-50-bha17136028","provider":"BioHippo","version":"1.0","type":"link"}