{"product_id":"timm10-antibody-tim10-mitochondrial-import-inner-membrane-translocase-subunit-tim10-bha17136041","title":"TIMM10 Antibody \/ TIM10 \/ Mitochondrial import inner membrane translocase subunit Tim10","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTIMM10 Antibody \/ TIM10 \/ Mitochondrial import inner membrane translocase subunit Tim10 is a anti-TIMM10 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TIMM10\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTIMM10 antibody detects Mitochondrial import inner membrane translocase subunit Tim10, a small chaperone protein essential for protein transport across the mitochondrial inner membrane. The UniProt recommended name is Mitochondrial import inner membrane translocase subunit Tim10 (TIMM10). Also known as Tim10, this protein is a central component of the TIM22 complex, which imports and assembles metabolite carrier proteins into the inner membrane.\u003cbr\u003e\u003cbr\u003eFunctionally, TIMM10 antibody identifies a 103-amino-acid protein residing in the mitochondrial intermembrane space. TIMM10 forms a hexameric complex with TIMM9 to guide hydrophobic precursor proteins through the aqueous intermembrane environment to the TIM22 translocase. This chaperone-mediated import system maintains mitochondrial protein homeostasis and bioenergetic function.\u003cbr\u003e\u003cbr\u003eThe TIMM10 gene is located on chromosome 11q13.1 and is expressed in energy-demanding tissues such as heart, brain, and skeletal muscle. TIMM10 ensures proper import of metabolite carriers, including the ADP\/ATP translocase and phosphate carrier, which are critical for mitochondrial metabolism.\u003cbr\u003e\u003cbr\u003ePathologically, defects in TIMM10 or its partner subunits lead to mitochondrial protein import deficiencies and oxidative phosphorylation defects. Impaired TIMM10 function can contribute to neurodegenerative and metabolic diseases. Research using TIMM10 antibody supports studies in mitochondrial biology, protein import, and bioenergetics.\u003cbr\u003e\u003cbr\u003eTIMM10 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect mitochondrial translocase components. \u003cbr\u003e\u003cbr\u003eStructurally, Mitochondrial import inner membrane translocase subunit Tim10 adopts a twin CX3C motif stabilized by disulfide bonds, forming a compact structure for substrate chaperoning. This antibody enables investigation of TIMM10's role in mitochondrial protein import and inner membrane assembly.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TIMM10 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TIMM10 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TIMM10 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TIMM10 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TIMM10+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047313367405,"sku":"FY13139","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e386892a-f9a2-43e5-86f7-16fe1319d1ab.jpg?v=1782237101","url":"https:\/\/www.ebiohippo.com\/products\/timm10-antibody-tim10-mitochondrial-import-inner-membrane-translocase-subunit-tim10-bha17136041","provider":"BioHippo","version":"1.0","type":"link"}