{"product_id":"timm8b-antibody-tim8-b-bha17135662","title":"TIMM8B Antibody \/ Tim8 B","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTIMM8B Antibody \/ Tim8 B is a anti-TIMM8B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TIMM8B\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTIMM8B antibody detects Mitochondrial import inner membrane translocase subunit Tim8B, a small chaperone-like protein that assists in importing and assembling hydrophobic carrier proteins into the mitochondrial inner membrane. Encoded by the TIMM8B gene on chromosome 11q14.1, this protein belongs to the mitochondrial intermembrane space (IMS) chaperone complex family, which also includes Tim8A, Tim9, and Tim10. TIMM8B forms part of the TIM8-TIM13 complex that stabilizes and delivers precursor proteins across the IMS to the TIM22 translocase complex, ensuring proper mitochondrial biogenesis and energy metabolism.\u003cbr\u003e\u003cbr\u003eStructurally, TIMM8B contains conserved twin CX3C motifs that coordinate zinc ions, stabilizing its folding and interaction with partner subunits. Within the IMS, TIMM8B associates with TIMM13 to form a 70 kilodalton heterohexameric complex. This assembly binds and shuttles hydrophobic transmembrane segments of carrier proteins, preventing their aggregation during translocation. Loss or dysfunction of TIMM8B disrupts mitochondrial protein import, leading to defects in oxidative phosphorylation and metabolic balance. While TIMM8A mutations cause Mohr-Tranebjaerg syndrome, TIMM8B plays a complementary and partially compensatory role in mitochondrial maintenance.\u003cbr\u003e\u003cbr\u003eThe TIMM8B antibody is widely used in cell biology, mitochondrial, and neurodegeneration research to study protein import mechanisms and mitochondrial function. Western blot analysis identifies a 10 kilodalton band corresponding to TIMM8B, while immunofluorescence reveals punctate mitochondrial localization that colocalizes with markers such as TOM20 and COX IV. This antibody enables precise examination of mitochondrial translocase assembly and protein trafficking. Because mitochondrial dysfunction contributes to neurodegenerative disorders, TIMM8B detection provides valuable insights into pathophysiology and energy metabolism.\u003cbr\u003e\u003cbr\u003eBeyond its role in import, TIMM8B contributes to mitochondrial quality control and stress response by maintaining the stability of the carrier import pathway. It supports the biogenesis of metabolite transporters such as ANT, MPC, and phosphate carriers. The TIMM8B antibody supports detailed analysis of these pathways and is validated by\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TIMM8B - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TIMM8B - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TIMM8B - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TIMM8B - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TIMM8B+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047298949485,"sku":"FY12759","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_17e7e3c7-94ab-40bb-b6e9-107cf85eef5a.jpg?v=1782237041","url":"https:\/\/www.ebiohippo.com\/products\/timm8b-antibody-tim8-b-bha17135662","provider":"BioHippo","version":"1.0","type":"link"}