{"product_id":"tlr3-minus-minus-sting-minus-minus-crispr-knockout-df-1-stable-cell-line-bhc10923295","title":"TLR3 \u003csup\u003e-\/-\u003c\/sup\u003eSTING \u003csup\u003e-\/-\u003c\/sup\u003e CRISPR Knockout DF-1 Stable Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eTlr3 \u003csup\u003e-\/-\u003c\/sup\u003esting \u003csup\u003e-\/-\u003c\/sup\u003e\u003c\/em\u003e has been disrupted in the  (Chicken (\u003cem\u003eGallus gallus\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of Tlr3 \u003csup\u003e-\/-\u003c\/sup\u003esting \u003csup\u003e-\/-\u003c\/sup\u003e in embryo tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, fibroblast monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eTlr3 \u003csup\u003e-\/-\u003c\/sup\u003esting \u003csup\u003e-\/-\u003c\/sup\u003e\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; confirmed by sanger sequencing of frameshift-inducing indels\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDesign notes:\u003c\/strong\u003e The TLR3 -\/- STING -\/- CRISPR Knockout DF-1 Cell Line is a dual knockout model developed from the DF-1 chicken embryonic fibroblast cell line, featuring stable CRISPR\/Cas9-mediated disruption of both \u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e  — Chicken (\u003cem\u003eGallus gallus\u003c\/em\u003e), embryo origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eTlr3 \u003csup\u003e-\/-\u003c\/sup\u003esting \u003csup\u003e-\/-\u003c\/sup\u003e\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with embryo biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Tlr3%20\u0026lt;sup\u0026gt;-\/-\u0026lt;\/sup\u0026gt;sting%20\u0026lt;sup\u0026gt;-\/-\u0026lt;\/sup\u0026gt;\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=Tlr3%20\u0026lt;sup\u0026gt;-\/-\u0026lt;\/sup\u0026gt;sting%20\u0026lt;sup\u0026gt;-\/-\u0026lt;\/sup\u0026gt;+Chicken\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eTlr3 \u003csup\u003e-\/-\u003c\/sup\u003esting \u003csup\u003e-\/-\u003c\/sup\u003e\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003ePriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS (*Regular) + 1% Penicillin\/Streptomycin Solution (G255), 39.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, fibroblast\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eEmbryo\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eChicken (\u003cem\u003eGallus gallus\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Confirmed by Sanger Sequencing of frameshift-inducing INDELs.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eTlr3 \u003csup\u003e-\/-\u003c\/sup\u003esting \u003csup\u003e-\/-\u003c\/sup\u003e\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental  line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by Tlr3 \u003csup\u003e-\/-\u003c\/sup\u003esting \u003csup\u003e-\/-\u003c\/sup\u003e using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eTlr3 \u003csup\u003e-\/-\u003c\/sup\u003esting \u003csup\u003e-\/-\u003c\/sup\u003e\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental  cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: Tlr3 \u003csup\u003e-\/-\u003c\/sup\u003esting \u003csup\u003e-\/-\u003c\/sup\u003e | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Tlr3 \u003csup\u003e-\/-\u003c\/sup\u003esting \u003csup\u003e-\/-\u003c\/sup\u003e\n- UniProt: Tlr3 \u003csup\u003e-\/-\u003c\/sup\u003esting \u003csup\u003e-\/-\u003c\/sup\u003e | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=Tlr3 \u003csup\u003e-\/-\u003c\/sup\u003esting \u003csup\u003e-\/-\u003c\/sup\u003e\n- abm product page | abmgood.com\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10923295\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53192378319213,"sku":"T6586","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/0f8winmihS5DlkHh1KCyvE1tbrHuFu0NeNiuIBoI.png?v=1775226767","url":"https:\/\/www.ebiohippo.com\/products\/tlr3-minus-minus-sting-minus-minus-crispr-knockout-df-1-stable-cell-line-bhc10923295","provider":"BioHippo","version":"1.0","type":"link"}