{"product_id":"tmc6-antibody-transmembrane-channel-like-protein-6-bha17135316","title":"TMC6 Antibody \/ Transmembrane channel-like protein 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTMC6 Antibody \/ Transmembrane channel-like protein 6 is a anti-TMC6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus membrane, Golgi.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TMC6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe TMC6 antibody targets Transmembrane channel-like protein 6, a multipass membrane protein encoded by the TMC6 gene. Also known as EVER1, this protein is involved in zinc homeostasis and antiviral defense, particularly in epithelial and keratinocyte cells. Transmembrane channel-like protein 6 forms a complex with TMC8 (EVER2) to regulate intracellular zinc distribution, influencing transcriptional activity and immune responses. The TMC6 antibody provides a reliable reagent for studying epithelial immunity, metal ion regulation, and susceptibility to viral infection.\u003cbr\u003e\u003cbr\u003eTransmembrane channel-like protein 6 is localized mainly to the endoplasmic reticulum and nuclear envelope, where it contributes to the regulation of zinc-dependent transcription factors. The TMC6 antibody supports localization and expression analyses that reveal how zinc flux affects keratinocyte differentiation and immune signaling. Through its partnership with TMC8, it restricts viral replication, particularly for human papillomaviruses (HPVs), by maintaining proper zinc balance within the nucleus.\u003cbr\u003e\u003cbr\u003eMutations in TMC6 cause Epidermodysplasia verruciformis (EV), a rare genodermatosis characterized by chronic HPV infection and increased skin cancer risk. The TMC6 antibody supports studies of this disease by enabling detection of protein loss or mislocalization in affected tissues. Lack of functional Transmembrane channel-like protein 6 disrupts zinc homeostasis, leading to impaired immune defense against HPV and uncontrolled keratinocyte proliferation. This discovery has highlighted TMC6 as a crucial host factor for viral resistance in epithelial tissues.\u003cbr\u003e\u003cbr\u003eBeyond antiviral defense, Transmembrane channel-like protein 6 influences immune cell signaling and skin barrier function. The TMC6 antibody aids in quantifying these effects, revealing how altered zinc regulation impacts inflammation and epidermal differentiation. Its expression is highest in stratified epithelia but may also occur in immune cells and other tissues involved in zinc transport and immune surveillance.\u003cbr\u003e\u003cbr\u003eThe TMC6 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence, providing distinct perinuclear staining consistent with endoplasmic reticulum localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TMC6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TMC6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TMC6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TMC6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TMC6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288889709,"sku":"FY12413","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b33cdcc3-b46e-4243-ae48-c88f2be91522.jpg?v=1782237015","url":"https:\/\/www.ebiohippo.com\/products\/tmc6-antibody-transmembrane-channel-like-protein-6-bha17135316","provider":"BioHippo","version":"1.0","type":"link"}