{"product_id":"tmed1-antibody-transmembrane-emp24-domain-containing-protein-1-bha17135347","title":"TMED1 Antibody \/ Transmembrane emp24 domain-containing protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTMED1 Antibody \/ Transmembrane emp24 domain-containing protein 1 is a anti-TMED1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TMED1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTMED1 antibody detects transmembrane emp24 domain-containing protein 1, a member of the p24 family of cargo receptors that regulate protein trafficking between the endoplasmic reticulum (ER) and Golgi apparatus. TMED1 participates in vesicle formation and quality control of secretory and membrane proteins, ensuring that correctly folded proteins are transported while misfolded ones are retained or degraded. The p24 family functions as part of the COPI and COPII coat protein systems, playing critical roles in maintaining organelle integrity and trafficking efficiency. The TMED1 antibody is an important reagent for studying vesicular transport, protein sorting, and ER-Golgi dynamics in various cell types.\u003cbr\u003e\u003cbr\u003eTMED1 is encoded by the TMED1 gene located on human chromosome 19p13.3. The protein comprises a luminal GOLD (Golgi dynamics) domain, a single transmembrane helix, and a short cytoplasmic tail that interacts with coatomer components. TMED1 has been implicated in the trafficking of toll-like receptors and other immune-related proteins, highlighting its role in innate immune signaling. In the Golgi network, TMED1 contributes to the formation of transport vesicles and sorting of glycoproteins, making it a crucial element in secretory pathway organization.\u003cbr\u003e\u003cbr\u003eThrough use of the TMED1 antibody, researchers have identified strong expression in the Golgi apparatus and ER exit sites, where TMED1 co-localizes with other p24 family members such as TMED2, TMED4, and TMED10. Western blot typically reveals a band near 25-27 kDa corresponding to the full-length protein. Immunofluorescence labeling with this antibody provides a distinct perinuclear staining pattern consistent with Golgi localization. TMED1 expression has been linked to protein homeostasis, and its dysregulation may contribute to cellular stress responses and unfolded protein response activation.\u003cbr\u003e\u003cbr\u003eRecent studies suggest TMED1's involvement in autophagy and viral protein processing, expanding its relevance beyond classical ER-Golgi transport. Knockdown or knockout experiments have shown altered secretion of select cytokines and membrane receptors, emphasizing its importance in vesicle biogenesis and immune communication.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TMED1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TMED1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TMED1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TMED1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TMED1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288693101,"sku":"FY12444","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8ed7c716-1a58-4b12-a706-aaf1b5c5be0f.jpg?v=1782237003","url":"https:\/\/www.ebiohippo.com\/products\/tmed1-antibody-transmembrane-emp24-domain-containing-protein-1-bha17135347","provider":"BioHippo","version":"1.0","type":"link"}