{"product_id":"tmed8-antibody-transmembrane-emp24-domain-containing-protein-8-bha17136216","title":"TMED8 Antibody \/ Transmembrane emp24 domain-containing protein 8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTMED8 Antibody \/ Transmembrane emp24 domain-containing protein 8 is a anti-TMED8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TMED8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTMED8 antibody detects Transmembrane emp24 domain-containing protein 8, a membrane-associated trafficking protein encoded by the TMED8 gene on chromosome 14q11.2. TMED8 is a member of the p24 cargo receptor family, which plays a key role in protein transport between the endoplasmic reticulum (ER) and Golgi apparatus. The TMED8 protein localizes to the ER-Golgi intermediate compartment (ERGIC) and Golgi membranes, where it regulates vesicle formation, cargo selection, and quality control of secretory proteins. Structurally, TMED8 contains a luminal GOLD (Golgi dynamics) domain, a coiled-coil region, a single transmembrane helix, and a short cytoplasmic tail harboring motifs for coat protein complex interaction.\u003cbr\u003e\u003cbr\u003eTMED8 antibody identifies a protein that contributes to the maintenance of Golgi structure and trafficking efficiency. The p24 family proteins, including TMED8, function as cargo receptors that facilitate selective packaging of client proteins into COPII-coated vesicles at the ER membrane. They also participate in retrograde transport via COPI vesicles, ensuring the recycling of resident ER enzymes and chaperones. TMED8 specifically regulates the trafficking of glycoproteins and receptor complexes involved in cell signaling and immune response.\u003cbr\u003e\u003cbr\u003eAt the cellular level, TMED8 is localized predominantly to the endomembrane system, reflecting its function in the secretory pathway. Through its luminal GOLD domain, TMED8 mediates protein-protein interactions that stabilize cargo-receptor complexes, ensuring proper folding and export from the ER. Structural studies of the p24 family suggest that TMED8 forms oligomeric complexes with other p24 members, such as TMED2, TMED9, and TMED10, enhancing vesicular trafficking specificity.\u003cbr\u003e\u003cbr\u003eFunctionally, TMED8 has been implicated in immune regulation and inflammatory signaling. It is known to interact with components of the TGF-beta receptor pathway, influencing receptor stability and cell surface expression. Dysregulation of TMED8 expression may lead to impaired protein secretion, ER stress, and altered immune responses. In addition, emerging studies suggest a role for TMED8 in tumorigenesis, where aberrant expression correlates with altered cytokine signaling and cell adhesion. Gene expression analyses show TMED8 upregulation in certain cancers, including breast and colon carcinoma, linking it to enhanced secretory pathway activity.\u003cbr\u003e\u003cbr\u003eThe TMED8 gene�s chromosomal location on 14q11.2 places it near other trafficking-related genes, and its promoter region includes binding sites for transcription factors involved in ER stress response. Evolutionarily, TMED8 belongs to the TMED\/p24 protein family, a conserved group essential for vesicular transport and Golgi organization. Defects in this pathway can lead to congenital disorders of glycosylation and impaired immune signaling.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using TMED8 antibody shows membrane and perinuclear localization consistent with Golgi and ER distribution, particularly in hepatocytes, epithelial cells, and macrophages. TMED8 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TMED8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TMED8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TMED8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TMED8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TMED8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047322050925,"sku":"FY13314","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5a9a4d4a-d3d7-4576-9c70-3af67a077ad1.jpg?v=1782237115","url":"https:\/\/www.ebiohippo.com\/products\/tmed8-antibody-transmembrane-emp24-domain-containing-protein-8-bha17136216","provider":"BioHippo","version":"1.0","type":"link"}