{"product_id":"tmpo-antibody-thymopoietin-bha17135581","title":"TMPO Antibody \/ Thymopoietin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTMPO Antibody \/ Thymopoietin is a anti-TMPO Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TMPO\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTMPO antibody recognizes Thymopoietin, a nuclear envelope-associated protein that plays crucial roles in nuclear structure organization, chromatin dynamics, and cell cycle regulation. TMPO exists in multiple isoforms generated by alternative splicing, including LAP2-alpha, LAP2-beta, and LAP2-gamma, which localize to distinct nuclear compartments and perform specialized functions. LAP2-beta and LAP2-gamma are integral components of the inner nuclear membrane that bind Lamin proteins, contributing to nuclear lamina stability, whereas LAP2-alpha remains nucleoplasmic and participates in chromatin assembly and gene regulation. The TMPO gene, located on chromosome 12q22, encodes these isoforms that collectively maintain nuclear architecture and influence genome organization.\u003cbr\u003e\u003cbr\u003eDuring cell division, TMPO isoforms coordinate nuclear envelope breakdown and reassembly, interacting with Lamins A\/C and chromatin-associated proteins such as BAF (Barrier-to-Autointegration Factor). The interaction between TMPO and Lamin A\/C is essential for post-mitotic nuclear reformation and for anchoring chromatin to the nuclear periphery. Dysregulation of TMPO expression or mutation disrupts lamina organization, leading to nuclear morphology defects observed in laminopathies and premature aging syndromes. Beyond its structural roles, TMPO also influences cell proliferation and differentiation by modulating E2F-dependent transcription and histone deacetylase activity.\u003cbr\u003e\u003cbr\u003eThe TMPO antibody is used to detect Thymopoietin and its LAP2 isoforms in various experimental systems, including western blotting, immunofluorescence, and immunohistochemistry. It enables investigation of nuclear envelope integrity, chromatin positioning, and protein-protein interactions involving the nuclear lamina. In disease research, TMPO has been studied for its role in cancer cell cycle regulation and chromatin organization abnormalities. Its involvement in maintaining genomic stability makes it a valuable marker for nuclear envelope dynamics.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TMPO - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TMPO - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TMPO - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TMPO - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TMPO+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296426349,"sku":"FY12678","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4a463387-6b98-4ab7-bbe4-1f5e95b53054.jpg?v=1782237026","url":"https:\/\/www.ebiohippo.com\/products\/tmpo-antibody-thymopoietin-bha17135581","provider":"BioHippo","version":"1.0","type":"link"}