{"product_id":"tns3-antibody-tensin-3-bha17135727","title":"TNS3 Antibody \/ Tensin 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTNS3 Antibody \/ Tensin 3 is a anti-TNS3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm, cell junctions.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TNS3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTNS3 antibody detects Tensin-3, a cytoskeletal adaptor protein that links integrins to the actin cytoskeleton and participates in cell adhesion, migration, and signal transduction. Encoded by the TNS3 gene on chromosome 7p12.3, this protein is part of the tensin family, which plays key roles in focal adhesion dynamics and mechanical signal integration. Tensin-3 contributes to the structural connection between integrin-mediated extracellular matrix adhesion sites and intracellular actin filaments, regulating cellular shape and motility.\u003cbr\u003e\u003cbr\u003eStructurally, Tensin-3 contains Src homology 2 (SH2) and phosphotyrosine-binding (PTB) domains that interact with phosphorylated tyrosine residues on focal adhesion proteins and with integrin cytoplasmic tails. Through these domains, TNS3 facilitates recruitment of signaling molecules, including Src family kinases, PI3K, and FAK, at adhesion sites. It also modulates Rho GTPase signaling, influencing actin organization and cell polarity.\u003cbr\u003e\u003cbr\u003eThe TNS3 antibody is widely used in cancer biology, cytoskeletal research, and cell signaling studies to investigate adhesion remodeling and integrin signaling. Western blot analysis detects a 150-160 kilodalton band corresponding to Tensin-3, while immunofluorescence reveals focal adhesion and cytoplasmic localization. This antibody supports studies examining adhesion turnover, migration, and mechanotransduction pathways in normal and malignant cells.\u003cbr\u003e\u003cbr\u003eTensin-3 expression is frequently altered in cancer, where it influences metastatic potential and invasion by regulating focal adhesion stability and actin remodeling. Overexpression promotes invasive behavior in several carcinoma types, while loss of function impairs migration and cell spreading. The TNS3 antibody provides a reliable reagent for exploring adhesion-mediated signaling and tumor cell motility.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TNS3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TNS3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TNS3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TNS3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TNS3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303045485,"sku":"FY12825","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_eaecf142-b77a-4d0a-97c0-094064341593.jpg?v=1782237063","url":"https:\/\/www.ebiohippo.com\/products\/tns3-antibody-tensin-3-bha17135727","provider":"BioHippo","version":"1.0","type":"link"}