{"product_id":"topbp1-antibody-dna-topoisomerase-ii-binding-protein-1-bha17135996","title":"TOPBP1 Antibody \/ DNA topoisomerase II-binding protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTOPBP1 Antibody \/ DNA topoisomerase II-binding protein 1 is a anti-TOPBP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TOPBP1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IP, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTOPBP1 antibody detects DNA topoisomerase II-binding protein 1, a nuclear scaffold protein essential for DNA replication initiation, checkpoint activation, and repair. The UniProt recommended name is DNA topoisomerase II-binding protein 1 (TOPBP1). This large BRCT domain-containing protein acts as a mediator between replication machinery and checkpoint kinases ATR and CHK1 to maintain genomic stability.\u003cbr\u003e\u003cbr\u003eFunctionally, TOPBP1 antibody identifies a 1522-amino-acid nuclear protein that binds DNA, replication origins, and repair complexes. TOPBP1 activates ATR kinase during replication stress, recruiting it to single-stranded DNA regions coated with replication protein A (RPA). It also coordinates DNA damage repair by interacting with RAD9-RAD1-HUS1 and other checkpoint complexes, ensuring proper S-phase progression.\u003cbr\u003e\u003cbr\u003eThe TOPBP1 gene is located on chromosome 3q22.1 and is expressed in proliferating cells across many tissues. It plays key roles in origin firing by recruiting DNA polymerase alpha\/primase complexes and regulating topoisomerase II at replication forks. Through its multiple BRCT domains, TOPBP1 serves as a dynamic hub for DNA replication and checkpoint signaling.\u003cbr\u003e\u003cbr\u003ePathologically, overexpression or mutation of TOPBP1 has been linked to genomic instability, cancer progression, and chemoresistance. Increased TOPBP1 expression is observed in breast, ovarian, and lung cancers, where it supports high replication demand and stress tolerance. Its checkpoint-regulating activity makes it a potential target for sensitizing tumors to DNA-damaging therapies. Research using TOPBP1 antibody provides insight into replication stress response and genome surveillance mechanisms.\u003cbr\u003e\u003cbr\u003eTOPBP1 antibody is suitable for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect nuclear replication complexes. \u003cbr\u003e\u003cbr\u003eStructurally, TOPBP1 contains eight BRCT domains that recognize phosphorylated protein motifs, mediating interactions with DNA repair factors and replication initiators. This antibody helps elucidate how TOPBP1 integrates replication initiation and checkpoint control to preserve genome integrity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TOPBP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TOPBP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TOPBP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TOPBP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TOPBP1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312122221,"sku":"FY13094","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9a9294be-3a50-4897-a38f-9b7f763b7a17.jpg?v=1782237099","url":"https:\/\/www.ebiohippo.com\/products\/topbp1-antibody-dna-topoisomerase-ii-binding-protein-1-bha17135996","provider":"BioHippo","version":"1.0","type":"link"}