{"product_id":"total-histone-h3-acetylation-detection-kit-fluorometric-bht19600003","title":"Total Histone H3 Acetylation Detection Kit (Fluorometric)","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;border:1px solid #ccd9f0}.bhp-desc td{padding:8px 12px;border:1px solid #ccd9f0}.bhp-desc .pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc .warn{background:#fff6ee;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"pill\"\u003eAssay Kit\u003c\/span\u003e\u003cp\u003eHistone H3 acetylation involves the addition of acetyl groups to lysine residues on histone proteins, which neutralizes their positive charge and relaxes chromatin structure, facilitating gene transcription, which are associated with DNA in chromatin. Histone acetylation is regulated by histone acetyltransferases (HATs), which add acetyl groups, and histone deacetylases (HDACs), which remove them. Histone H3 acetylation is tightly involved in cell cycle regulation, cell proliferation, and apoptosis. Disruption of histone acetylation balance in cancer cells, linked to abnormal transcription factor binding, promotes tumorigenesis, cancer progression, and metastasis. The Total Histone H3 Acetylation Detection Assay is designed to measure histone H3 acetylation in various mammalian cells, including human and mouse cells, as well as fresh and frozen tissues, cultured adherent cells, and suspension cells. This assay is simple, user-friendly, accurate, and fast. The measurement can be completed in 2.5 hours, with a recommended sample input of 5 ng to 2 µg per well. Alternative Name: H3ac\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAlternative names:\u003c\/strong\u003e H3ac\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThe Total Histone H3 Acetylation Detection Kit (Fluorometric) is a fluorometric immunoassay for the quantitative measurement of acetylated histone h3 in mammalian cells and tissues. Target histones are captured on a pre-coated microplate and detected with a modification-specific antibody, then quantified against a standard curve. The assay measures total histone h3 acetylation with a recommended sample input of 5 ng – 2 µg per well and can be completed in 2.5 hours.\u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cp\u003eThe kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (modification-specific antibody and enzyme\/fluorophore conjugate), wash buffer, and developing\/stop solutions. Refer to the product datasheet for the full component list and volumes.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Target\u003c\/th\u003e\n\u003ctd\u003eTotal histone H3 acetylation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnalyte\u003c\/th\u003e\n\u003ctd\u003eAcetylated Histone H3 (total)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eFluorometric immunoassay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eFluorometric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Time\u003c\/th\u003e\n\u003ctd\u003e2.5 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecommended Sample Input\u003c\/th\u003e\n\u003ctd\u003e5 ng – 2 µg per well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eHuman, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Compatibility\u003c\/th\u003e\n\u003ctd\u003eMammalian cells (human, mouse); fresh \u0026amp; frozen tissues; adherent \u0026amp; suspension cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore the unopened kit at 2–8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"warn\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices and consult the SDS before handling.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"1 x 96-well plate","offer_id":53550126465389,"sku":"TBS32122","price":690.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TBS32122-1.jpg?v=1784060060","url":"https:\/\/www.ebiohippo.com\/products\/total-histone-h3-acetylation-detection-kit-fluorometric-bht19600003","provider":"BioHippo","version":"1.0","type":"link"}