{"product_id":"total-rna-extraction-kit-bht15200026","title":"Total RNA Extraction Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRNA Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe reagents used in this kit do not contain phenol and chloroform, which greatly reduces the harm of phenol and chloroform to the experimenter and expands the use environment. This product can quickly extract total RNA from animal cells, tissues, and can process a large number of different samples simultaneously. Extracted total RNA has high purity and extremely low contamination of proteins and other impurities, which can be used for RT-PCR, Real Time RT-PCR, microarray analysis, Northern Blot, Dot Blot, poly(A) selection, in vitro translation, RNase protection analysis and molecular cloning, and various other downstream experiments.\u003c\/p\u003e\n\u003cp\u003eSamples are lysed in 300 µL of Lysate REL, digested with 10 µL of Proteinase K in 590 µL of ddH₂O at 56°C for 10 min and clarified at 12,000 rpm for 5 min. After adding 0.5 volumes of ethanol the lysate is loaded onto Adsorption column R, washed with 750 µL of Buffer RRPB and then twice with 500 µL of Washing Buffer RWB, dried by centrifugation at 12,000 rpm for 2 min followed by air-drying, and eluted in 30–100 µL of RNase-Free ddH₂O. An optional on-column DNase I step is included. For pure RNA the OD260\/OD280 reading is between 1.8 and 2.1, and 28S rRNA is about twice that of 18S rRNA.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSpin column (Adsorption column R)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample types\u003c\/th\u003e\n\u003ctd\u003eAnimal cells and tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e20 mg tissue, or adherent or suspended cells lysed in 300 µL of lysate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eChemistry\u003c\/th\u003e\n\u003ctd\u003ePhenol- and chloroform-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDNase I treatment\u003c\/th\u003e\n\u003ctd\u003eOptional on-column step; DNase I stock solution and DNase I Buffer RDB supplied\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity (OD260\/OD280)\u003c\/th\u003e\n\u003ctd\u003eBetween 1.8 and 2.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRNA integrity\u003c\/th\u003e\n\u003ctd\u003e28S rRNA is about twice that of 18S rRNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e30–100 µL RNase-Free ddH₂O (minimum 30 µL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003cth\u003e200 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLysate REL\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNA deproteinized Buffer RRPB\u003c\/td\u003e\n\u003ctd\u003e70 mL\u003c\/td\u003e\n\u003ctd\u003e140 mL × 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWashing Buffer RWB\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003ctd\u003e240 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteinase K\u003c\/td\u003e\n\u003ctd\u003e525 µL\u003c\/td\u003e\n\u003ctd\u003e2.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDNase I stock solution\u003c\/td\u003e\n\u003ctd\u003e525 µL\u003c\/td\u003e\n\u003ctd\u003e2.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDNase I Buffer RDB\u003c\/td\u003e\n\u003ctd\u003e5 mL\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdsorption column R\u003c\/td\u003e\n\u003ctd\u003e50 sets\u003c\/td\u003e\n\u003ctd\u003e200 sets\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-Free ddH₂O\u003c\/td\u003e\n\u003ctd\u003e40 mL\u003c\/td\u003e\n\u003ctd\u003e160 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTotal RNA extraction from animal cells and tissues\u003c\/li\u003e\n\u003cli\u003eRT-PCR and Real Time RT-PCR\u003c\/li\u003e\n\u003cli\u003eNorthern Blot and Dot Blot analysis\u003c\/li\u003e\n\u003cli\u003eMicroarray analysis and poly(A) selection\u003c\/li\u003e\n\u003cli\u003eIn vitro translation, RNase protection analysis and molecular cloning\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eHigh purity: The purified total RNA has very low protein contamination, and the obtained RNA can be used for Northern blot, dot blot, mRNA extraction, cDNA synthesis, primer extension, difference display, etc.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eLysate REL, Buffer RRPB, Washing Buffer RWB, DNase I Buffer RDB, Adsorption column R and RNase-Free ddH₂O are stored at room temperature for 1 year. Proteinase K and the DNase I stock solution are stored at -20°C for 1 year.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAbsolute ethanol must be added to Washing Buffer RWB before use.\u003c\/li\u003e\n\u003cli\u003eExcessive tissue affects lysis efficiency; increase the lysate proportionally if needed.\u003c\/li\u003e\n\u003cli\u003eComplete drying of the adsorption material is critical, otherwise residues interfere with downstream experiments.\u003c\/li\u003e\n\u003cli\u003eThe elution volume should not be below 30 µL, as it affects recovery efficiency.\u003c\/li\u003e\n\u003cli\u003eStore the eluted RNA at -70°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP014\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200026\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"50 T","offer_id":53965512573293,"sku":"EP014-50T","price":140.4,"currency_code":"USD","in_stock":true},{"title":"200 T","offer_id":53965514342765,"sku":"EP014-200T","price":390.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 10","offer_id":53965514375533,"sku":"EP014-50TX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"200 T x 5","offer_id":53965514408301,"sku":"EP014-200TX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP014-a-1.jpg?v=1789419185","url":"https:\/\/www.ebiohippo.com\/products\/total-rna-extraction-kit-bht15200026","provider":"BioHippo","version":"1.0","type":"link"}