{"product_id":"tox1-reporter-lentivirus-bhv19400231","title":"TOX1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eTOX (thymocyte selection-associated high mobility group box protein) is a DNA-binding factor of the HMG-box family that influences gene regulation and chromatin organization. TOX is essential for normal T cell development, particularly thymocyte selection, and it acts as a key driver of the T cell exhaustion program. In response to chronic antigen stimulation, sustained TOX expression promotes the differentiation of exhausted T cells, reshaping their transcriptional and epigenetic state. Because exhausted T cells limit antitumor and antiviral immunity, TOX has become an important factor in immunology and immuno-oncology research, and reporters of its activity help characterize T cell differentiation.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe TOX1 Reporter Lentivirus is a transcription-factor reporter system for detecting TOX1-mediated transcriptional activity. It contains tandem repeats of consensus TOX1 DNA-binding elements, coupled to a minimal TATA-box promoter and an upstream enhancer that maximizes signal-to-noise to drive a fluorescent or luminescent reporter (including BFP2, d2GFP, EGFP, GFP, mCherry, RFP, firefly, Gaussia, or Renilla luciferase). A constitutively expressed selection marker (blasticidin, hygromycin, puromycin, or zeocin) supports stable polyclonal cell line generation. Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved material, with readout by microscopy, flow cytometry, or luminometry. It is used to study TOX1 activity in T cell development, exhaustion, and differentiation. Supplied as purified lentiviral particles.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the TOX1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"TOX1-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251625091437,"sku":"LTV-0124-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362084741485,"sku":"LTV-0124-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362084774253,"sku":"LTV-0124-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"TOX1-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362084807021,"sku":"LTV-0124-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362084839789,"sku":"LTV-0124-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362084872557,"sku":"LTV-0124-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362084905325,"sku":"LTV-0124-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"TOX1-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362084938093,"sku":"LTV-0124-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"TOX1-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362084970861,"sku":"LTV-0124-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"TOX1-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362085003629,"sku":"LTV-0124-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"TOX1-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362085036397,"sku":"LTV-0124-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TOX1-Reporter-Figure.jpg?v=1776934220","url":"https:\/\/www.ebiohippo.com\/products\/tox1-reporter-lentivirus-bhv19400231","provider":"BioHippo","version":"1.0","type":"link"}