{"product_id":"tox3-antibody-tox-high-mobility-group-box-family-member-3-bha17135939","title":"TOX3 Antibody \/ TOX high mobility group box family member 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTOX3 Antibody \/ TOX high mobility group box family member 3 is a anti-TOX3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TOX3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTOX3 antibody detects TOX high mobility group box family member 3, a nuclear transcriptional cofactor involved in calcium-dependent gene regulation, chromatin remodeling, and neuronal survival. The UniProt recommended name is TOX high mobility group box family member 3 (TOX3). This protein functions as a chromatin-modifying transcriptional regulator and is expressed primarily in brain tissue, where it contributes to synaptic maintenance and neuroprotection.\u003cbr\u003e\u003cbr\u003eFunctionally, TOX3 antibody identifies a 576-amino-acid nuclear protein containing a high mobility group (HMG) box domain that binds to DNA and alters chromatin structure. TOX3 acts as a transcriptional co-regulator by interacting with CREB-binding protein (CBP), CITED1, and other transcriptional activators, enhancing the expression of calcium-dependent genes. It modulates neuronal plasticity and calcium signaling pathways crucial for learning, memory, and neuroprotection.\u003cbr\u003e\u003cbr\u003eThe TOX3 gene is located on chromosome 16q12.1 and encodes a transcription factor expressed in central nervous system neurons, endocrine tissues, and certain epithelial cells. Through chromatin remodeling, TOX3 facilitates access of transcriptional machinery to DNA and influences the balance between gene activation and repression. Its HMG box domain induces DNA bending, promoting nucleosome repositioning and chromatin flexibility.\u003cbr\u003e\u003cbr\u003eTOX3 has attracted attention as a genetic susceptibility locus in breast cancer, where specific polymorphisms near the TOX3 gene are associated with increased risk. In tumors, aberrant TOX3 expression can alter transcriptional programs governing cell differentiation and apoptosis. In the nervous system, TOX3 helps protect neurons from excitotoxicity by modulating calcium-regulated gene networks. Reduced expression has been linked to increased vulnerability to neurodegenerative injury and impaired neuronal survival.\u003cbr\u003e\u003cbr\u003eTOX3 antibody is widely used in neuroscience, epigenetics, and cancer biology research. It is suitable for western blotting, immunohistochemistry, and chromatin immunoprecipitation to detect TOX3 expression and DNA binding activity. This antibody supports studies of transcriptional regulation, calcium signaling, and chromatin dynamics. In oncology, it aids in exploring the functional relevance of TOX3 variants in hormone-responsive cancers.\u003cbr\u003e\u003cbr\u003eStructurally, TOX3 contains an HMG DNA-binding domain and transcriptional regulatory regions that interact with coactivators and histone acetyltransferases. Its activity is modulated by calcium-dependent phosphorylation and nuclear localization signals.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TOX3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TOX3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TOX3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TOX3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TOX3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309402477,"sku":"FY13037","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_dff1a3c5-d13e-452d-8b5e-6d4f8c862642.jpg?v=1782237067","url":"https:\/\/www.ebiohippo.com\/products\/tox3-antibody-tox-high-mobility-group-box-family-member-3-bha17135939","provider":"BioHippo","version":"1.0","type":"link"}