{"product_id":"tpbg-antibody-trophoblast-glycoprotein-bha17135364","title":"TPBG Antibody \/ Trophoblast glycoprotein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTPBG Antibody \/ Trophoblast glycoprotein is a anti-TPBG Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TPBG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTPBG antibody detects Trophoblast glycoprotein, a highly glycosylated cell surface protein involved in cell adhesion, signal transduction, and immune regulation. Also known as 5T4 oncofetal antigen, TPBG is a type I transmembrane glycoprotein expressed in trophoblasts and upregulated in many carcinomas. The TPBG antibody is used extensively in cancer biology, immunology, and stem cell research to study tumor invasion, metastasis, and cell differentiation mechanisms regulated by this protein.\u003cbr\u003e\u003cbr\u003eTPBG is encoded by the TPBG gene located on human chromosome 6q14.1. The mature protein is approximately 420 amino acids long and features an N-terminal signal peptide, several leucine-rich repeats, and an immunoglobulin-like domain in its extracellular region. It is anchored to the plasma membrane through a single transmembrane helix and contains a short cytoplasmic tail. Physiologically, TPBG modulates cell-cell interactions in placental development and embryo implantation, while pathologically, it is re-expressed in many epithelial tumors, where it contributes to epithelial-mesenchymal transition (EMT) and metastatic behavior.\u003cbr\u003e\u003cbr\u003eThe TPBG antibody is commonly used in immunohistochemistry and flow cytometry to identify tumor cells expressing the 5T4 antigen. Elevated TPBG expression has been reported in lung, ovarian, colorectal, and pancreatic cancers and is associated with poor prognosis and increased invasiveness. The protein influences Wnt\/beta-catenin and CXCR4 signaling pathways, promoting migration and stem-like phenotypes. Western blot typically shows a broad band between 72-80 kDa, reflecting heavy glycosylation. Immunofluorescence microscopy reveals localization along the plasma membrane and at cell junctions.\u003cbr\u003e\u003cbr\u003eIn stem cell research, TPBG expression marks the transition from pluripotency to differentiation, making it a useful biomarker in embryonic stem cell studies. It modulates cytoskeletal dynamics and cell polarity through interactions with intracellular adaptors. Therapeutically, TPBG serves as a target for cancer immunotherapies, including antibody-drug conjugates and vaccines under clinical evaluation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TPBG - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TPBG - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TPBG - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TPBG - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TPBG+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289282925,"sku":"FY12461","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_cdbacb09-e3fc-43b0-bd69-27e9361dc975.jpg?v=1782237005","url":"https:\/\/www.ebiohippo.com\/products\/tpbg-antibody-trophoblast-glycoprotein-bha17135364","provider":"BioHippo","version":"1.0","type":"link"}