{"product_id":"tpcn2-antibody-two-pore-channel-2-bha17135633","title":"TPCN2 Antibody \/ Two-pore channel 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTPCN2 Antibody \/ Two-pore channel 2 is a anti-TPCN2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TPCN2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTPCN2 antibody detects Two-pore channel 2, an endolysosomal cation channel that mediates calcium and sodium flux in response to nicotinic acid adenine dinucleotide phosphate (NAADP) signaling. Encoded by the TPCN2 gene on chromosome 11q13.4, this channel is part of the two-pore channel family that regulates intracellular calcium release from acidic organelles such as lysosomes and endosomes. TPCN2 contains two homologous pore-forming domains, each with six transmembrane helices, and functions as a homodimeric voltage-gated ion channel. It plays a key role in vesicle trafficking, autophagy, endosomal fusion, and cellular excitability.\u003cbr\u003e\u003cbr\u003eTPCN2 is widely expressed in kidney, liver, pancreas, and smooth muscle tissues, where it coordinates calcium-dependent signaling events. Activation of TPCN2 by NAADP triggers localized calcium release that modulates downstream pathways, including mTOR signaling, lysosomal exocytosis, and metabolic adaptation. Genetic studies have linked TPCN2 variants to pigmentation differences and hair color determination, highlighting its broader physiological influence. In neurons, TPCN2 regulates endolysosomal dynamics essential for synaptic vesicle recycling and signal transmission.\u003cbr\u003e\u003cbr\u003eThe TPCN2 antibody is a key reagent for cell signaling, membrane biology, and neurophysiology studies. Western blot analysis identifies a 75 kilodalton band corresponding to full-length TPCN2, while immunofluorescence reveals punctate staining consistent with lysosomal localization. The antibody enables detection of TPCN2 in subcellular fractionation and colocalization experiments with LAMP1 and Rab7 markers. Because endolysosomal calcium release impacts autophagy, metabolism, and exocytosis, this antibody supports research into pathways controlling intracellular trafficking and energy homeostasis.\u003cbr\u003e\u003cbr\u003eLoss or dysregulation of TPCN2 impairs lysosomal function, alters autophagic flux, and contributes to disorders involving calcium signaling imbalance. Elevated expression has been associated with cancer cell invasiveness and altered vesicular pH homeostasis. The TPCN2 antibody allows researchers to evaluate how NAADP-dependent calcium signaling influences physiological and pathological processes.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TPCN2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TPCN2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TPCN2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TPCN2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TPCN2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047298130285,"sku":"FY12730","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_62435ede-c9df-4757-b974-9310af5c2bf2.jpg?v=1782237033","url":"https:\/\/www.ebiohippo.com\/products\/tpcn2-antibody-two-pore-channel-2-bha17135633","provider":"BioHippo","version":"1.0","type":"link"}