{"product_id":"tph2-antibody-tryptophan-hydroxylase-2-bha17135945","title":"TPH2 Antibody \/ Tryptophan hydroxylase 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTPH2 Antibody \/ Tryptophan hydroxylase 2 is a anti-TPH2 Rabbit antibody Recombinant Rabbit Monoclonal clone 31T01 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TPH2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31T01, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTPH2 antibody detects Tryptophan hydroxylase 2, encoded by the TPH2 gene. Tryptophan hydroxylase 2 is the rate limiting enzyme in the biosynthesis of serotonin in the brain. By catalyzing the hydroxylation of tryptophan to 5 hydroxytryptophan, which is then decarboxylated to serotonin, this enzyme plays an essential role in neurotransmission, mood regulation, and circadian rhythms. TPH2 antibody provides researchers with a vital tool to study serotonin metabolism, neuronal signaling, and psychiatric disorders.\u003cbr\u003e\u003cbr\u003eTryptophan hydroxylase 2 is distinct from TPH1, which performs the same enzymatic reaction but is expressed primarily in peripheral tissues. TPH2 is enriched in serotonergic neurons of the raphe nuclei in the brainstem. Research with TPH2 antibody has revealed that disruption of this enzyme reduces central serotonin production, leading to alterations in behavior, mood, and stress responses. This central role has made TPH2 a focus of studies in depression, anxiety, and other mood disorders.\u003cbr\u003e\u003cbr\u003eMutations in TPH2 have been associated with psychiatric disease and developmental disorders. Genetic variants that reduce enzyme activity have been linked to susceptibility to depression, bipolar disorder, and autism spectrum disorders. Studies using TPH2 antibody in postmortem brain tissue have confirmed altered expression in individuals with psychiatric conditions, supporting its clinical relevance. Animal models with disrupted TPH2 expression also display behavioral phenotypes, reinforcing the causal role of this enzyme in serotonin dependent pathways.\u003cbr\u003e\u003cbr\u003eBeyond psychiatry, TPH2 is relevant in circadian biology, stress physiology, and drug response. Serotonin produced by TPH2 serves as a precursor for melatonin synthesis in the pineal gland, linking it to circadian regulation. Research with TPH2 antibody has shown that stress hormones modulate its expression, suggesting integration of environmental cues with neurotransmitter metabolism. Because many antidepressants and antipsychotics target serotonin pathways, TPH2 remains an important pharmacological research target.\u003cbr\u003e\u003cbr\u003eTPH2 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting measures enzyme levels in brain lysates, immunohistochemistry demonstrates expression in raphe nuclei and other serotonergic structures, and immunofluorescence allows visualization of neuronal localization. These methods make TPH2 antibody indispensable in neuroscience research.\u003cbr\u003e\u003cbr\u003eBy providing validated TPH2 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TPH2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TPH2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TPH2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TPH2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TPH2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047309992301,"sku":"FY13043","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d4edd3c8-744e-47a0-a8ec-685cf3e2c239.jpg?v=1782237068","url":"https:\/\/www.ebiohippo.com\/products\/tph2-antibody-tryptophan-hydroxylase-2-bha17135945","provider":"BioHippo","version":"1.0","type":"link"}