{"product_id":"trail-r3-antibody-dcr1-bha17135876","title":"TRAIL R3 Antibody \/ DcR1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTRAIL R3 Antibody \/ DcR1 is a anti-R3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31T28 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e R3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31T28, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTRAIL R3 antibody detects Decoy receptor 1, encoded by the TNFRSF10C gene. This protein is also referred to as TRAIL receptor 3 and DcR1, and it belongs to the tumor necrosis factor receptor superfamily. Unlike signaling TRAIL receptors, DcR1 lacks a functional cytoplasmic death domain and therefore does not induce apoptosis. Instead, it acts as a decoy receptor by binding TRAIL (tumor necrosis factor related apoptosis inducing ligand) and preventing it from activating apoptotic pathways through receptors such as TRAIL R1 and TRAIL R2. TRAIL R3 antibody provides a tool for researchers to investigate how apoptosis is regulated in normal tissues and tumors.\u003cbr\u003e\u003cbr\u003eDecoy receptor 1 is expressed in many normal tissues, particularly in the gastrointestinal tract, lungs, and liver, where it may help protect against inappropriate TRAIL mediated apoptosis. Studies using TRAIL R3 antibody have shown that its presence prevents excessive cell death, preserving tissue integrity under inflammatory conditions. In immune regulation, DcR1 may act as a safeguard, balancing the cytotoxic effects of TRAIL expressing immune cells with the need to maintain healthy tissue survival.\u003cbr\u003e\u003cbr\u003eIn cancer biology, expression of Decoy receptor 1 has been reported in multiple tumor types. By competing for TRAIL binding, DcR1 enables malignant cells to resist TRAIL induced apoptosis, thereby promoting survival. Research with TRAIL R3 antibody has demonstrated that tumors expressing high levels of DcR1 show decreased sensitivity to TRAIL based therapies. This has made TNFRSF10C a focus of interest in understanding resistance mechanisms to immunotherapy and targeted apoptosis inducing drugs.\u003cbr\u003e\u003cbr\u003eTRAIL R3 antibody is useful in western blotting, immunohistochemistry, and flow cytometry. Western blotting confirms expression in cell lines and tissues, while immunohistochemistry demonstrates distribution across normal and cancerous tissues. Flow cytometry with TRAIL R3 antibody allows analysis of surface expression on live cells, aiding in functional studies of TRAIL signaling. These applications provide insights into how DcR1 balances death receptor signaling in health and disease.\u003cbr\u003e\u003cbr\u003eBy providing validated TRAIL R3 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=R3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=R3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=R3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/R3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=R3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047308058989,"sku":"FY12974","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_504eb257-9399-4f09-b64f-8a52b33e32d7.jpg?v=1772019370","url":"https:\/\/www.ebiohippo.com\/products\/trail-r3-antibody-dcr1-bha17135876","provider":"BioHippo","version":"1.0","type":"link"}