{"product_id":"tram-34-bhb21300335","title":"TRAM 34","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e 1-[(2-Chlorophenyl)diphenylmethyl]-1H-pyrazole.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlso known as:\u003c\/strong\u003e TRAM-34\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 289905-88-0\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C22H17ClN2.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;99%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 10 nM - 1 μM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized Powder\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e DMSO. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. Soluble in any conventional buffer containing 0.01 KH2PO4, 0.1% gelatin and\/or 0.1% bovine serum albumin (BSA). The use of gelatin or 0.01% BSA coated\/treated glassware is recommended. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e KCa3.1 K+ channels\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Synthetic\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e TRAM 34 is a potent and selective KCa3.1 (SK4, intermediate conductance Ca2+-activated K+) channel inhibitor1. As such, it is a powerful pharmacological tool that enables the determination of the roles played by KCa3.1 channels in different physiological systems ranging from activation of T lymphocytes to migration of endothelial cells2-3.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlternative names:\u003c\/strong\u003e TRAM-34\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003eKCa3.1 (SK4, intermediate conductance Ca2+ activated K+) is part of the Ca2+ activated K+ channel family that share the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is six transmembrane domains and intracellular N- and C-termini. KCa3.1 is widely expressed in epithelial, endothelial cells and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel1.TRAM 34 is a potent and selective KCa3.1 channel inhibitor; 50 nM blocks KCa3.1 channel expressed in COS-7 cells by over 50%2. As such, it is a powerful pharmacological tool that enables the determination of the roles played by KCa3.1 channels in different physiological systems, ranging from activation of T lymphocytes to migration of endothelial cells3-4.By using TRAM 34 it was demonstrated that proliferation of vascular endothelial cells and angiogenesis in vivo are mediated via KCa3.1 channels4.in vivo delivery of TRAM 34 via balloon catheter prevented porcine coronary smooth muscle phenotypic modulation and limited subsequent vasculoproliferative diseases5.10 mM TRAM 34, completely stopped proliferation of the human pancreatic cancer cell lines, (BxPC-3 MiaPaCa-2) while proliferation of PANC-1 cells was hardly affected by TRAM 34, which was well correlated to the elevated levels of KCa3.1 expression in the former as well as in samples from pancreatic cancer patients6.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e \u003ch3\u003eCited application(s)\u003c\/h3\u003e \u003cp\u003eElectrophysiology\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"10 mg \/ 1","offer_id":53249714651501,"sku":"T-105-10MG-1","price":110.0,"currency_code":"USD","in_stock":true},{"title":"25 mg \/ 1","offer_id":53311577784685,"sku":"T-105-25MG-1","price":250.0,"currency_code":"USD","in_stock":true},{"title":"5 mg \/ 1","offer_id":53311577817453,"sku":"T-105-5MG-1","price":81.0,"currency_code":"USD","in_stock":true},{"title":"50 mg \/ 1","offer_id":53311577850221,"sku":"T-105-50MG-1","price":306.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/T-105_gr.gif?v=1778544453","url":"https:\/\/www.ebiohippo.com\/products\/tram-34-bhb21300335","provider":"BioHippo","version":"1.0","type":"link"}