{"product_id":"translin-antibody-tsn-bha17136269","title":"Translin Antibody \/ TSN","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTranslin Antibody \/ TSN is a anti-TSN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Nucleus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TSN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTranslin antibody detects Translin, a highly conserved RNA and DNA-binding protein encoded by the TSN gene located on chromosome 2q11.2. Translin functions as part of the RNA processing and transport machinery, forming a complex with Trax (TSNAX) to create the C3PO (component 3 promoter of RNA-induced silencing complex) ribonuclease. This complex plays a key role in the RNA interference (RNAi) pathway by promoting the removal of passenger strands from small interfering RNAs (siRNAs), thereby facilitating gene silencing. Translin is ubiquitously expressed, with high levels found in neurons, testes, and lymphoid tissues where RNA transport and regulation are crucial.\u003cbr\u003e\u003cbr\u003eStructurally, Translin assembles into an octameric ring that binds nucleic acids in a sequence-independent manner. It contains a central RNA\/DNA-binding domain and regions that mediate oligomerization and association with Trax. Together, Translin and Trax form the C3PO complex, which promotes RNA-induced silencing by accelerating siRNA duplex unwinding. Translin belongs to the Translin family of RNA-binding proteins that regulate post-transcriptional processes across multiple tissues.\u003cbr\u003e\u003cbr\u003eFunctionally, Translin acts in mRNA transport, localization, and stabilization. In neurons, it regulates axonal mRNA transport and local translation critical for synaptic plasticity. It also participates in DNA double-strand break repair and chromosomal translocation control, linking RNA metabolism to genome maintenance. Through the C3PO complex, Translin contributes to RNAi efficiency and microRNA (miRNA) processing, ensuring proper post-transcriptional gene regulation. Known binding partners include TSNAX (Trax), AGO2, and Dicer, all involved in RNA interference and processing.\u003cbr\u003e\u003cbr\u003eTranslin expression is developmentally regulated, peaking during neuronal maturation and spermatogenesis. In germ cells, it contributes to mRNA trafficking essential for gametogenesis. In immune cells, it regulates cytokine mRNA stability and turnover. Dysregulation of Translin has been linked to neurodevelopmental disorders and cancers, where aberrant RNA regulation and chromosomal rearrangements contribute to disease progression. Pathway associations include RNA transport, RNA interference, and DNA damage repair signaling.\u003cbr\u003e\u003cbr\u003eThe Translin antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TSN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TSN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TSN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TSN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TSN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323328877,"sku":"FY13367","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_28ce3ab7-0078-4eb1-92d8-22572fe7142f.jpg?v=1782237107","url":"https:\/\/www.ebiohippo.com\/products\/translin-antibody-tsn-bha17136269","provider":"BioHippo","version":"1.0","type":"link"}