{"product_id":"trim40-antibody-tripartite-motif-containing-protein-40-bha17136191","title":"TRIM40 Antibody \/ Tripartite motif-containing protein 40","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTRIM40 Antibody \/ Tripartite motif-containing protein 40 is a anti-TRIM40 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TRIM40\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTRIM40 antibody detects Tripartite motif-containing protein 40, a member of the TRIM family of E3 ubiquitin ligases that regulate immune signaling, protein degradation, and cellular homeostasis. The TRIM40 gene encodes a cytosolic protein characterized by a RING finger domain, one or two B-box motifs, and a coiled-coil region, which together constitute the tripartite motif typical of this protein family. TRIM40 plays a crucial role in modulating NF-kappaB and interferon signaling pathways, acting as a negative regulator of innate immune responses.\u003cbr\u003e\u003cbr\u003eBiochemical studies have demonstrated that TRIM40 mediates ubiquitination and degradation of components within the NF-kappaB pathway, including IKK gamma (NEMO), thereby dampening inflammatory signaling. It is expressed predominantly in gastrointestinal epithelial cells and immune tissues, where it contributes to immune tolerance and inflammation control. TRIM40 also regulates Rho GTPase signaling, maintaining cytoskeletal integrity and epithelial barrier function. Reduced expression or dysregulation of TRIM40 has been associated with inflammatory bowel disease, colorectal cancer, and autoimmune conditions, suggesting its role as a protective modulator of mucosal immunity.\u003cbr\u003e\u003cbr\u003eLocated on chromosome 6p21.33 within the MHC class I region, the TRIM40 gene is inducible by cytokines and microbial stimuli. The protein's E3 ligase activity enables it to tag target proteins for proteasomal degradation, ensuring tight control of inflammatory pathways. In addition to immune regulation, TRIM40 may influence oncogenic processes by modulating NF-kappaB-driven transcription of genes related to proliferation and survival. Overexpression studies indicate that TRIM40 stabilizes cytoskeletal structures and promotes cell adhesion, counteracting tumor cell invasiveness.\u003cbr\u003e\u003cbr\u003eImmunostaining using TRIM40 antibody demonstrates cytoplasmic localization in intestinal epithelium and lymphoid tissues. The antibody serves as a valuable reagent for exploring immune signaling, epithelial cell regulation, and tumor suppression mechanisms. TRIM40 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TRIM40 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TRIM40 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TRIM40 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TRIM40 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TRIM40+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047320936813,"sku":"FY13289","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_771e4780-406f-4904-8237-27ac2bbb264e.jpg?v=1782237083","url":"https:\/\/www.ebiohippo.com\/products\/trim40-antibody-tripartite-motif-containing-protein-40-bha17136191","provider":"BioHippo","version":"1.0","type":"link"}