{"product_id":"trim44-antibody-tripartite-motif-containing-protein-44-bha17135821","title":"TRIM44 Antibody \/ Tripartite motif-containing protein 44","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTRIM44 Antibody \/ Tripartite motif-containing protein 44 is a anti-TRIM44 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TRIM44\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTRIM44 antibody detects Tripartite motif-containing protein 44, an E3 ubiquitin ligase-like protein that regulates cell growth, stress response, and signaling through its role in ubiquitination and protein stabilization. Encoded by the TRIM44 gene on chromosome 11p13, this protein belongs to the tripartite motif (TRIM) family, characterized by a RING finger domain, B-box motifs, and a coiled-coil region that mediate protein-protein interactions. TRIM44 functions as a scaffold protein that influences autophagy, signal transduction, and oncogenic pathways by regulating substrate ubiquitination.\u003cbr\u003e\u003cbr\u003eStructurally, TRIM44 is a 512-amino-acid cytoplasmic protein of approximately 57 kilodaltons containing a zinc-binding B-box and coiled-coil domains typical of TRIM proteins. Unlike many family members, TRIM44 lacks a canonical RING finger domain but instead contains a zinc metalloprotease-like region that mediates protein stabilization rather than degradation. This structural divergence enables TRIM44 to protect target proteins from proteasomal degradation, thereby modulating multiple signaling cascades including NF-?B and PI3K\/AKT pathways.\u003cbr\u003e\u003cbr\u003eThe TRIM44 antibody is widely used in cancer biology, immunology, and cell signaling research to study ubiquitin-dependent protein regulation, tumor progression, and cellular stress response. Western blot analysis detects a 57 kilodalton band corresponding to TRIM44, while immunofluorescence shows cytoplasmic and perinuclear staining, reflecting its role in signaling and vesicular trafficking. This antibody provides a dependable reagent for investigating the molecular mechanisms by which TRIM44 regulates protein turnover and signal transduction.\u003cbr\u003e\u003cbr\u003eFunctionally, TRIM44 stabilizes key signaling proteins including mTOR and NF-?B regulators, promoting survival under stress conditions such as oxidative damage or nutrient deprivation. Overexpression of TRIM44 has been observed in multiple cancers including gastric, lung, and breast carcinomas, where it enhances proliferation, migration, and epithelial-mesenchymal transition (EMT). Conversely, its depletion sensitizes cells to apoptosis and autophagic degradation. The TRIM44 antibody supports research exploring ubiquitin signaling networks, autophagy regulation, and cancer-associated proteostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TRIM44 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TRIM44 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TRIM44 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TRIM44 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TRIM44+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307043181,"sku":"FY12919","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c6e5765a-7f81-432a-966a-20c72e8cbc9a.jpg?v=1782237065","url":"https:\/\/www.ebiohippo.com\/products\/trim44-antibody-tripartite-motif-containing-protein-44-bha17135821","provider":"BioHippo","version":"1.0","type":"link"}