{"product_id":"trim59-antibody-tripartite-motif-containing-protein-59-bha17135358","title":"TRIM59 Antibody \/ Tripartite motif-containing protein 59","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTRIM59 Antibody \/ Tripartite motif-containing protein 59 is a anti-TRIM59 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: ER.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TRIM59\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTRIM59 antibody recognizes Tripartite motif-containing protein 59, an E3 ubiquitin ligase that regulates diverse cellular processes including innate immunity, oncogenic signaling, and protein stability. TRIM59 belongs to the TRIM family characterized by a RING finger domain, one or two B-box domains, and a coiled-coil region responsible for self-association and substrate recognition. As a member of this family, TRIM59 modulates ubiquitination and degradation of target proteins, influencing signal transduction, apoptosis, and transcriptional regulation. The TRIM59 antibody is widely used to investigate tumorigenesis, immune signaling, and ubiquitin-proteasome pathway regulation.\u003cbr\u003e\u003cbr\u003eTRIM59 is encoded by the TRIM59 gene on human chromosome 3q25.33. The protein localizes mainly to the cytoplasm but can translocate to the nucleus under specific signaling conditions. It interacts with multiple oncogenic and tumor-suppressive pathways, including p53, STAT3, and Ras-mediated cascades. Overexpression of TRIM59 has been documented in various cancers such as gastric, lung, and breast carcinoma, where it promotes cell proliferation, invasion, and resistance to apoptosis.\u003cbr\u003e\u003cbr\u003eStudies using the TRIM59 antibody have revealed that TRIM59 acts as an oncogenic factor by promoting ubiquitin-dependent degradation of p53, thereby suppressing apoptosis and enhancing tumor growth. Additionally, TRIM59 participates in innate immune signaling by modulating the STING and NF-kB pathways, influencing cytokine production during viral infection. Western blot analysis typically shows a band at approximately 42-48 kDa. Immunofluorescence using this antibody reveals diffuse cytoplasmic staining, occasionally enriched in perinuclear compartments.\u003cbr\u003e\u003cbr\u003eTRIM59 functions as part of a larger regulatory network controlling inflammation and autophagy. It has been associated with epigenetic modulation through interactions with chromatin-remodeling factors, affecting transcriptional activation of oncogenes. In cancer models, TRIM59 depletion suppresses tumor cell migration and enhances p53-dependent growth inhibition.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TRIM59 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TRIM59 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TRIM59 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TRIM59 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TRIM59+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288594797,"sku":"FY12455","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_30a8e569-6d65-4ca4-8b8a-42fbf4327fbf.jpg?v=1782237015","url":"https:\/\/www.ebiohippo.com\/products\/trim59-antibody-tripartite-motif-containing-protein-59-bha17135358","provider":"BioHippo","version":"1.0","type":"link"}