{"product_id":"tripure-total-rna-extraction-reagent-bht15200027","title":"TRIpure Total RNA Extraction Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eExtraction Reagents\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eTRIpure Total RNA Extraction Reagent is a reagent for total RNA extraction from various animal and plant, bacterial tissues\/cells. It lyses cells and tissue rapidly and keeps the RNA in the sample intact, effectively inhibiting RNA degradation. The sample is lysed completely in the reagent. After centrifugation with chloroform, the solution forms a supernatant layer, an intermediate layer and an organic layer (lower pink layer). The RNA is distributed in the upper aqueous phase, and the supernatant layer is collected. Total RNA is recovered by isopropanol precipitation. The extracted total RNA is of high purity, with low protein and genomic DNA carryover. It can be directly used for Northern, dot hybridization, mRNA purification, in vitro translation, RT-PCR, poly(A)+ selection, RNase protection analysis, and construction of cDNA libraries and a variety of other molecular biology experiments.\u003c\/p\u003e\n\u003cp\u003eTRIpure is a phenol-based reagent. One mL of TRIpure handles adherent cells from a 10 cm² culture area, 5×10⁶–1×10⁷ suspended cells, 10⁷ bacteria, 200 µL whole blood, 30–100 mg animal tissue or 50–100 mg plant tissue. The upper aqueous layer recovered after chloroform separation is about 50–60% of the total amount of added TRIpure. For pure RNA the OD260\/OD280 ratio is between 1.9 and 2.2. Chloroform, isopropyl alcohol, 75% ethanol (DEPC water), DEPC and RNase-free equipment are supplied by the user. Per sample, 300 µL of chloroform is added and shaken for 15 s, the phases are separated by centrifugation at 12,000 rpm, the aqueous phase is precipitated with an equal volume of isopropanol at -20°C for 20 min, and the RNA pellet is washed with 75% ethanol and air-dried for 2–3 min.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSingle-component phenol-based liquid extraction reagent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample types\u003c\/th\u003e\n\u003ctd\u003eAnimal, plant and bacterial tissues and cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input per 1 mL reagent\u003c\/th\u003e\n\u003ctd\u003eAdherent cells from 10 cm² culture area, 5×10⁶–1×10⁷ suspended cells, 10⁷ bacteria, 200 µL whole blood, 30–100 mg animal tissue or 50–100 mg plant tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity (OD260\/OD280)\u003c\/th\u003e\n\u003ctd\u003eBetween 1.9 and 2.2 for pure RNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAqueous phase recovered\u003c\/th\u003e\n\u003ctd\u003eAbout 50–60% of the TRIpure volume added\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003eTotal RNA extracted within 1 h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTotal RNA extraction from animal, plant and bacterial tissues and cells\u003c\/li\u003e\n\u003cli\u003eRNA extraction from virus and other trace samples\u003c\/li\u003e\n\u003cli\u003eNorthern blotting and dot hybridization\u003c\/li\u003e\n\u003cli\u003eRT-PCR and in vitro translation\u003c\/li\u003e\n\u003cli\u003emRNA purification, poly(A)+ selection and cDNA library construction\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHigh extraction quality: Total RNA with high yield, high purity and good integrity can be extracted within 1 h.\u003c\/li\u003e\n\u003cli\u003eColored indicator: a dye turns the lower layer pink after centrifugal layering, so the upper aqueous phase is easy to remove.\u003c\/li\u003e\n\u003cli\u003eHigh sensitivity: Higher extraction efficiency for the extraction of virus and other trace samples.\u003c\/li\u003e\n\u003cli\u003eFlexible sample handling capacity: It has a good lysis effect on a small amount of tissue (50–100 mg) and cells (5×10⁶) and a large number of tissues (≥1 g) and cells (\u0026gt;10⁷).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eThe reagent is stored at 4°C in the dark and is valid for 1 year. RNA precipitated in 75% ethanol keeps at 4°C for 1 week or at -20°C for 1 year. Shipping: Ice bag transportation.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePhenol is toxic and corrosive; handle in a fume hood with gloves and eye protection.\u003c\/li\u003e\n\u003cli\u003eUse RNase-free equipment throughout the procedure.\u003c\/li\u003e\n\u003cli\u003eWear gloves, protective clothing and a mask, and avoid talking during the operation.\u003c\/li\u003e\n\u003cli\u003eThe RNA half-life is relatively short and RNA is easy to degrade.\u003c\/li\u003e\n\u003cli\u003eStore the RNA precipitate in 75% ethanol at 4°C for up to 1 week or at -20°C for up to 1 year.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP013\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200027\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"100 mL","offer_id":53965512180077,"sku":"EP013-100ML","price":140.4,"currency_code":"USD","in_stock":true},{"title":"250 mL","offer_id":53965515882861,"sku":"EP013-250ML","price":243.1,"currency_code":"USD","in_stock":true},{"title":"100 mL x 10","offer_id":53965515915629,"sku":"EP013-100MLX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"250 mL x 40","offer_id":53965515948397,"sku":"EP013-250MLX40","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP013-a-1.jpg?v=1789419192","url":"https:\/\/www.ebiohippo.com\/products\/tripure-total-rna-extraction-reagent-bht15200027","provider":"BioHippo","version":"1.0","type":"link"}