{"product_id":"trpv4-antibody-transient-receptor-potential-cation-channel-subfamily-v-member-4-bha17135651","title":"TRPV4 Antibody \/ Transient receptor potential cation channel subfamily V member 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTRPV4 Antibody \/ Transient receptor potential cation channel subfamily V member 4 is a anti-TRPV4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TRPV4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTRPV4 antibody detects Transient receptor potential cation channel subfamily V member 4, a calcium-permeable non-selective cation channel that responds to mechanical, osmotic, and thermal stimuli. Encoded by the TRPV4 gene on chromosome 12q24.11, this protein belongs to the vanilloid subfamily of transient receptor potential (TRP) channels and functions as a multimodal sensor integrating diverse physiological signals. TRPV4 channels are activated by moderate heat (27-35oC), hypotonic cell swelling, shear stress, and endogenous lipids such as epoxyeicosatrienoic acids. These channels play central roles in osmoregulation, mechanotransduction, nociception, and vascular physiology.\u003cbr\u003e\u003cbr\u003eTRPV4 forms tetrameric channel complexes at the plasma membrane, where calcium influx through the pore initiates downstream signaling cascades. It interacts with cytoskeletal and scaffolding proteins including PACSIN3, AKAP150, and actin-binding proteins to fine-tune mechanosensitivity. In epithelial tissues, TRPV4 regulates ciliary beating and barrier permeability. In sensory neurons, it mediates mechanical pain and contributes to thermal sensation. In vascular endothelial cells, TRPV4 activation triggers calcium-dependent vasodilation through nitric oxide release, influencing blood pressure regulation and flow adaptation.\u003cbr\u003e\u003cbr\u003eThe TRPV4 antibody is widely used in physiology, neuroscience, and cardiovascular research to detect TRPV4 expression and localization. Western blot analysis typically identifies a 98 kilodalton band corresponding to TRPV4, while immunofluorescence reveals membrane and cytoplasmic localization in epithelial and neuronal cells. This antibody allows detailed investigation into TRPV4-mediated calcium signaling and mechanical responsiveness across tissues. Dysregulation of TRPV4 function is associated with diseases such as neuropathic pain, pulmonary edema, skeletal dysplasias, and Charcot-Marie-Tooth neuropathy type 2C.\u003cbr\u003e\u003cbr\u003eMechanistically, TRPV4 operates as a polymodal integrator linking mechanical and chemical cues to calcium signaling and gene expression. Mutations that enhance or suppress TRPV4 channel activity disrupt normal ion homeostasis and cellular volume regulation. The TRPV4 antibody enables researchers to assess expression changes under osmotic, mechanical, or thermal stress, supporting mechanistic studies of signal transduction and cellular adaptation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TRPV4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TRPV4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TRPV4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TRPV4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TRPV4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299015021,"sku":"FY12748","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_71f5e111-538b-499c-9e17-754bf4022b7c.jpg?v=1782237044","url":"https:\/\/www.ebiohippo.com\/products\/trpv4-antibody-transient-receptor-potential-cation-channel-subfamily-v-member-4-bha17135651","provider":"BioHippo","version":"1.0","type":"link"}