{"product_id":"tslp-bhn20152512","title":"TSLP","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis GenCefe mRNA encodes \u003cstrong\u003eTSLP\u003c\/strong\u003e, a cytokines construct supplied for antibody validation and cell-based binding assays. The product is formulated as lyophilised, non-encapsulated RNA and is intended for use in cell-based research applications requiring transient protein expression with reduced immunogenicity.\u003c\/p\u003e\n\u003ch2\u003emRNA Construct Design\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5′ Cap:\u003c\/strong\u003e Cap1 (m7GpppNm) — co-transcriptionally added during in vitro transcription (IVT). Cap1 includes 2′-O-methylation at the first transcribed nucleotide, closely mimicking the cap structure found on endogenous mammalian mRNA and reducing recognition by innate immune sensors (e.g., IFIT1\/IFIT3).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eModified Nucleotides:\u003c\/strong\u003e 100% N1-methylpseudouridine (m1Ψ; N1-Me-Pseudo UTP) substitution for all uridine residues. m1Ψ modification reduces TLR7\/TLR8-mediated innate immune activation and PKR-driven translational suppression, resulting in improved protein expression in immunocompetent cells and primary cell types.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003ePoly(A) Tail:\u003c\/strong\u003e 100–120 nt — enzymatically polyadenylated. The poly(A) tail stabilises the 3′ terminus, supports poly(A)-binding protein (PABP) recruitment, and enhances ribosome recycling for efficient cap-dependent translation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5′ UTR:\u003c\/strong\u003e hHBA1 (hemoglobin subunit alpha 1 5′ UTR) — a well-characterised human UTR that supports efficient cap-dependent translation initiation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e3′ UTR:\u003c\/strong\u003e hHBA1 (hemoglobin subunit alpha 1 3′ UTR) — provides post-transcriptional stability and modulates mRNA decay kinetics.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSignal Peptide:\u003c\/strong\u003e No\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eProtein Tag:\u003c\/strong\u003e No\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCodon Optimisation:\u003c\/strong\u003e No (native human codon usage retained)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003emRNA Length:\u003c\/strong\u003e Provided upon order placement.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilised powder; reconstitute in DEPC-treated water as needed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis mRNA is supplied as non-encapsulated, lyophilised powder. Delivery vehicle selection (LNP, electroporation, lipofection) is at the discretion of the end user and should be optimised for the target cell type and application.\u003c\/p\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003eCytokines are a broad class of small secreted proteins that mediate intercellular communication within the immune system and across a range of tissue types. They include interleukins (ILs), interferons (IFNs), tumour necrosis factors (TNFs), and colony-stimulating factors (CSFs), each acting through cognate receptors to modulate immune activation, inflammation, haematopoiesis, and cell survival. mRNA-encoded cytokines are increasingly employed in immuno-oncology research as reference standards for neutralisation assays, antibody validation, receptor-binding studies, and bioassay development. Transient cytokine expression via mRNA avoids the chromosomal integration concerns associated with viral vector approaches and allows rapid, reversible functional evaluation.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eImmuno-oncology bioassay standards: Cytokine mRNAs expressed in reporter cell lines are used as reference materials for neutralising antibody potency assays and ELISA calibration in therapeutic antibody development.\u003c\/li\u003e\n  \u003cli\u003eCAR T-cell armoured constructs: mRNA-encoded cytokines (e.g., IL-15, IL-21) are being evaluated in combination with CAR constructs to improve T-cell persistence in solid tumour microenvironments.\u003c\/li\u003e\n  \u003cli\u003eInflammation modelling: Transient cytokine mRNA transfection allows controlled induction of inflammatory gene signatures in primary macrophages and dendritic cells for mechanistic pathway studies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eNeutralisation assay standards — mRNA-expressed cytokines used as reference materials for potency and neutralisation testing of therapeutic antibodies in reporter-cell or bioassay formats.\u003c\/li\u003e\n  \u003cli\u003eReceptor binding validation — cell-surface expressed cytokine receptors or secreted cytokine ligands used in SPR, flow cytometry, and ELISA-based binding studies.\u003c\/li\u003e\n  \u003cli\u003eImmune cell activation studies — transient cytokine mRNA expression in primary immune cells (T cells, macrophages, NK cells) to dissect signalling pathways and transcriptional responses.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eCytokine secretion levels from transfected cells may vary with mRNA dose, cell type, and transfection efficiency; always quantify secreted protein by ELISA or bioassay before use as a standard.\u003c\/li\u003e\n  \u003cli\u003eFor neutralisation assays, confirm that the mRNA-expressed cytokine is biologically active (e.g., STAT signalling reporter) before using it as a reference antigen.\u003c\/li\u003e\n  \u003cli\u003eCytokine-encoding mRNAs can activate innate immune pathways in sensitive primary cells despite N1-Me-Pseudo UTP modification; include matched negative control mRNA (e.g., EGFP mRNA) at equivalent doses.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"GenCefe Biotech","offers":[{"title":"20 ug","offer_id":53239632560493,"sku":"IR0067002","price":279.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53239646519661,"sku":"IR0067010","price":339.0,"currency_code":"USD","in_stock":true},{"title":"200 ug","offer_id":53239646552429,"sku":"IR0067020","price":399.0,"currency_code":"USD","in_stock":true},{"title":"500 ug","offer_id":53239646585197,"sku":"IR0067050","price":459.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53239646617965,"sku":"IR0067100","price":549.0,"currency_code":"USD","in_stock":true},{"title":"5 mg","offer_id":53239646650733,"sku":"IR0067500","price":1799.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/mRNA_8c34b5a0-6caa-4388-a636-ab3c77985086.png?v=1776677729","url":"https:\/\/www.ebiohippo.com\/products\/tslp-bhn20152512","provider":"BioHippo","version":"1.0","type":"link"}