{"product_id":"tslp-blocking-peptide-for-stj505920-antibody-stj505712-bhp15502236","title":"TSLP Blocking Peptide for STJ505920 antibody (STJ505712)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc ul{margin:0 0 14px;padding-left:22px}\n.bhp-desc li{margin-bottom:4px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin:4px 6px 10px 0}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px;display:inline-block;margin:3px 4px 3px 0}\n.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin:0 6px 10px 0}\n\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis synthetic \u003cstrong\u003eTSLP Blocking Peptide\u003c\/strong\u003e corresponds to the immunogen sequence used to generate the paired anti-TSLP antibody (\u003cstrong\u003eSTJ505920\u003c\/strong\u003e). Pre-absorbing the antibody with this peptide before use demonstrates epitope-specific binding and confirms that observed signals in Western blot, IHC, or IF are attributable to the target protein, not antibody cross-reactivity.\u003c\/p\u003e\n\u003ch2\u003eTarget \u0026amp; Specificity\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eUniProt ID\u003c\/th\u003e\n\u003ctd\u003eQ969D9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eImmunogen\u003c\/th\u003e\n\u003ctd\u003eSynthetic peptide taken within amino acid region 50-100 on human Thymic Stromal LymphoPoietin.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eImmunogen Region (aa)\u003c\/th\u003e\n\u003ctd\u003e50-100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003eThis blocking peptide is recommended for use in combination with TSLP antibody, STJ505920\u003c\/p\u003e\n\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLiquid form at 2.5mg\/ml concentration in PBS. Up to 5% DMSO can be added. Orders with \u0026gt;1mg can be supplied in lyophilized powder form, or in buffer of choice.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at-20°C for long term storage. Avoid freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidated Applications\u003c\/th\u003e\n\u003ctd\u003eImmunodepletion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003ePre-Absorption Specificity Protocol\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eDilute the anti-TSLP antibody to the recommended working concentration in assay buffer.\u003c\/li\u003e\n\u003cli\u003eAdd the blocking peptide at a 5–10× molar excess relative to the antibody. For typical IHC conditions (2 μg\/mL antibody): use 10–20 μg\/mL blocking peptide.\u003c\/li\u003e\n\u003cli\u003eIncubate at room temperature for 1 hour (or at 4 °C overnight) with gentle mixing.\u003c\/li\u003e\n\u003cli\u003eUse the pre-absorbed mixture as the antibody solution in your standard WB\/IHC\/IF protocol, run in parallel with a non-blocked antibody control.\u003c\/li\u003e\n\u003cli\u003eComplete loss of signal in the pre-absorbed lane confirms epitope-specific binding. A non-specific irrelevant peptide should be included as a negative control.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Intended Use\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eResearch Use Only.\u003c\/strong\u003e For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle in accordance with good laboratory practice.\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eIntended Use\u003c\/strong\u003eResearch Use Only (RUO)\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eVendor\u003c\/strong\u003eSt John's Laboratory Ltd\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eCatalog No.\u003c\/strong\u003eSTJ505712\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eUniProt\u003c\/strong\u003eQ969D9\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"St John's Laboratory Ltd","offers":[{"title":"250 ug","offer_id":53335909499245,"sku":"STJ505712-250","price":359.49,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/product-no-image_933ebcd0-b7a1-4a2a-ad52-192ddfa99c17.png?v=1778913313","url":"https:\/\/www.ebiohippo.com\/products\/tslp-blocking-peptide-for-stj505920-antibody-stj505712-bhp15502236","provider":"BioHippo","version":"1.0","type":"link"}