{"product_id":"tsr1-antibody-pre-rrna-processing-protein-tsr1-bha17135403","title":"TSR1 Antibody \/ Pre-rRNA-processing protein TSR1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTSR1 Antibody \/ Pre-rRNA-processing protein TSR1 is a anti-TSR1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TSR1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTSR1 antibody detects Pre-rRNA-processing protein TSR1 homolog, a ribosome biogenesis factor required for the maturation of the small ribosomal subunit (40S). TSR1 is an essential nucleolar protein that participates in the late stages of pre-18S rRNA processing, ensuring proper ribosome assembly and translation efficiency. The TSR1 antibody is used in studies of ribosome formation, RNA metabolism, and cellular growth control.\u003cbr\u003e\u003cbr\u003eTSR1 is encoded by the TSR1 gene located on human chromosome 17q25.3. The protein is approximately 88 kilodaltons and conserved across eukaryotes, underscoring its critical role in ribosome biogenesis. TSR1 associates with pre-ribosomal particles in the nucleolus and cytoplasm and functions during the transition of 90S to 40S ribosomal precursors. It acts as a structural scaffold that stabilizes pre-rRNA intermediates and recruits processing enzymes necessary for final 18S rRNA cleavage.\u003cbr\u003e\u003cbr\u003eThe TSR1 antibody typically detects an 88-90 kilodalton band on western blot and is useful for immunofluorescence studies showing nucleolar and cytoplasmic localization. Knockdown or mutation of TSR1 disrupts ribosome biogenesis, leading to impaired protein synthesis and activation of nucleolar stress pathways such as p53 stabilization. As a core assembly factor, TSR1 interacts with RIO kinases, NOB1, and other late-acting ribosome maturation proteins.\u003cbr\u003e\u003cbr\u003eIn addition to its canonical role in ribosome formation, TSR1 may contribute to cell cycle progression and proliferation through its control of global translation. Aberrant expression of TSR1 has been observed in several cancers, suggesting a link between elevated ribosome production and tumorigenesis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TSR1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TSR1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TSR1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TSR1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TSR1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290167661,"sku":"FY12500","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5bf07f9b-9fbe-4900-ada2-5255cef7d2ee.jpg?v=1782237004","url":"https:\/\/www.ebiohippo.com\/products\/tsr1-antibody-pre-rrna-processing-protein-tsr1-bha17135403","provider":"BioHippo","version":"1.0","type":"link"}