{"product_id":"ttll1-antibody-tubulin-tyrosine-ligase-like-protein-1-bha17135340","title":"TTLL1 Antibody \/ Tubulin-tyrosine ligase-like protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTTLL1 Antibody \/ Tubulin-tyrosine ligase-like protein 1 is a anti-TTLL1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TTLL1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe TTLL1 antibody targets Tubulin-tyrosine ligase-like protein 1, an enzyme encoded by the TTLL1 gene that catalyzes the polyglutamylation of tubulin. Tubulin-tyrosine ligase-like protein 1 modifies the C-terminal tails of alpha-tubulin, generating polyglutamate side chains that regulate microtubule stability, interactions with motor proteins, and axonal transport. The TTLL1 antibody enables researchers to study post-translational modification of microtubules and the functional specialization of the cytoskeleton in neuronal and ciliary systems.\u003cbr\u003e\u003cbr\u003eTubulin-tyrosine ligase-like protein 1 belongs to the tubulin tyrosine ligase-like family, a group of enzymes that mediate tubulin polyglutamylation and polyglycylation. It primarily acts on alpha-tubulin within stable microtubule arrays such as those found in neurons and cilia. The TTLL1 antibody supports visualization of enzyme expression in neuronal soma and axons, reflecting its enrichment in brain tissue and importance for neural function. Loss of TTLL1 leads to reduced tubulin polyglutamylation, defective neuronal transport, and abnormal ciliary movement.\u003cbr\u003e\u003cbr\u003ePolyglutamylation modulates the binding of microtubule-associated proteins (MAPs) and molecular motors including kinesin and dynein. The TTLL1 antibody provides a valuable reagent for analyzing this regulatory layer of cytoskeletal control. Dysregulation of TTLL1 activity contributes to neurodegenerative and ciliopathy phenotypes due to altered microtubule organization. TTLL1-dependent modification also influences mitotic spindle assembly and chromosome segregation.\u003cbr\u003e\u003cbr\u003eTubulin-tyrosine ligase-like protein 1 localizes to the cytoplasm and is often concentrated at centrosomes and ciliary bases. The TTLL1 antibody supports localization studies revealing its participation in microtubule-anchoring regions. Mutations in TTLL1 have been associated with human developmental disorders involving cerebellar atrophy and cognitive delay, reinforcing its essential role in neuronal microtubule regulation.\u003cbr\u003e\u003cbr\u003eThe TTLL1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, yielding distinct cytoplasmic and centrosomal staining.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TTLL1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TTLL1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TTLL1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TTLL1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TTLL1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287021933,"sku":"FY12437","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_92f9bb37-f50a-4634-86ea-9969a44c3ea8.jpg?v=1782237015","url":"https:\/\/www.ebiohippo.com\/products\/ttll1-antibody-tubulin-tyrosine-ligase-like-protein-1-bha17135340","provider":"BioHippo","version":"1.0","type":"link"}