{"product_id":"ttll5-antibody-tubulin-tyrosine-ligase-like-protein-5-bha17135635","title":"TTLL5 Antibody \/ Tubulin tyrosine ligase-like protein 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTTLL5 Antibody \/ Tubulin tyrosine ligase-like protein 5 is a anti-TTLL5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TTLL5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTTLL5 antibody detects Tubulin polyglutamylase TTLL5 (Tubulin tyrosine ligase-like protein 5), an enzyme that catalyzes post-translational glutamylation of alpha- and beta-tubulin, thereby regulating microtubule stability, motility, and interactions with associated proteins. Encoded by the TTLL5 gene on chromosome 14q24.3, this enzyme belongs to the TTLL family of tubulin-modifying enzymes responsible for adding glutamate side chains to tubulin and other cytoskeletal proteins. TTLL5 contains a tubulin-tyrosine ligase domain and a coiled-coil region that mediate enzymatic activity and substrate recognition. Through microtubule glutamylation, TTLL5 plays crucial roles in ciliary function, intracellular trafficking, and sensory perception.\u003cbr\u003e\u003cbr\u003eTTLL5 is expressed in multiple tissues, including retina, testis, and brain, where it supports specialized microtubule structures such as photoreceptor connecting cilia and sperm flagella. In photoreceptor cells, TTLL5 regulates the glutamylation of ciliary axonemes and outer segment proteins critical for vision. Mutations in TTLL5 cause inherited retinal dystrophies, including cone-rod dystrophy and Leber congenital amaurosis, due to defective ciliary transport and photoreceptor degeneration. In spermatozoa, TTLL5 is essential for flagellar stability and motility, and its absence leads to asthenozoospermia and male infertility.\u003cbr\u003e\u003cbr\u003eThe TTLL5 antibody is widely used in cell biology, neurobiology, and reproductive research to study microtubule post-translational modification and ciliary biology. Western blot analysis identifies a 130 kilodalton band corresponding to the full-length enzyme, while immunofluorescence reveals localization along ciliary and cytoplasmic microtubules. Researchers employ this antibody to investigate TTLL5-dependent pathways controlling axonemal function and intracellular transport. Dysregulation of tubulin glutamylation has been implicated in neurodegenerative diseases and ciliopathies, making TTLL5 an important molecular marker for cytoskeletal regulation.\u003cbr\u003e\u003cbr\u003eFunctionally, TTLL5 acts with other TTLL family members to maintain the dynamic balance between glutamylation and deglutamylation, which modulates motor protein activity and vesicular transport. The TTLL5 antibody facilitates the study of these processes and enables precise mapping of TTLL5 expression across tissues.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TTLL5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TTLL5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TTLL5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TTLL5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TTLL5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047297835373,"sku":"FY12732","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_99eb1a0b-78da-48e6-a8a0-5a8923726e46.jpg?v=1782237029","url":"https:\/\/www.ebiohippo.com\/products\/ttll5-antibody-tubulin-tyrosine-ligase-like-protein-5-bha17135635","provider":"BioHippo","version":"1.0","type":"link"}