{"product_id":"tymp-antibody-thymidine-phosphorylase-pd-ecgf-bha17136239","title":"TYMP Antibody \/ Thymidine phosphorylase \/ PD-ECGF","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTYMP Antibody \/ Thymidine phosphorylase \/ PD-ECGF is a anti-TYMP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TYMP\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTYMP antibody detects Thymidine phosphorylase, also known as Platelet-derived endothelial cell growth factor (PD-ECGF), an enzyme encoded by the TYMP gene located on chromosome 22q13.33. TYMP is a cytoplasmic enzyme that catalyzes the reversible phosphorolysis of thymidine to thymine and 2-deoxy-D-ribose-1-phosphate, functioning in nucleotide metabolism and angiogenic signaling. It is widely expressed in endothelial cells, macrophages, and platelets, with particularly high levels observed in tumor vasculature and regenerative tissues. TYMP plays a dual role as a metabolic enzyme and a pro-angiogenic factor, linking DNA salvage pathways with vascular growth regulation.\u003cbr\u003e\u003cbr\u003eAs a key enzyme in pyrimidine salvage metabolism, TYMP maintains nucleotide pool balance for DNA synthesis and repair. Beyond its catalytic role, extracellular TYMP functions as PD-ECGF, promoting endothelial cell migration, angiogenesis, and wound healing. TYMP activity increases during hypoxia and tissue regeneration, facilitating the formation of new capillaries in response to injury or ischemia. Co-localization studies show TYMP associating with endothelial integrins and extracellular matrix components in angiogenic tissues.\u003cbr\u003e\u003cbr\u003eStructurally, TYMP forms a homodimer with each subunit containing a thymidine-binding pocket and phosphate-binding residues essential for catalysis. It belongs to the thymidine phosphorylase family of pyrimidine salvage enzymes. The enzyme's catalytic activity also generates deoxyribose sugars that act as angiogenic mediators, linking metabolism to vascular signaling. TYMP interacts with molecules such as integrin alphavbeta3 and extracellular matrix proteins to enhance endothelial cell adhesion and migration.\u003cbr\u003e\u003cbr\u003eFunctionally, TYMP contributes to multiple biological processes, including angiogenesis, platelet activation, and oxidative stress response. Its expression is regulated by hypoxia-inducible factor 1-alpha (HIF-1alpha), cytokines, and growth factors such as VEGF and TNF-alpha. In cancer, TYMP expression is upregulated in tumor-associated macrophages and endothelial cells, promoting neovascularization and tumor progression. However, TYMP also exhibits cytotoxic effects in thymidine phosphorylase-deficient conditions by accumulating toxic thymidine metabolites, as seen in mitochondrial neurogastrointestinal encephalopathy (MNGIE).\u003cbr\u003e\u003cbr\u003eDysregulation of TYMP is clinically significant. Loss-of-function mutations cause MNGIE, a rare mitochondrial disorder characterized by gastrointestinal dysmotility, neuropathy, and leukoencephalopathy. Overexpression of TYMP is associated with poor prognosis in several cancers, including colorectal, breast, and gastric carcinoma, where it drives angiogenesis and tumor growth. Pathway involvement includes pyrimidine salvage metabolism, hypoxia response, and VEGF-mediated angiogenic signaling. In regenerative medicine, TYMP serves as a biomarker and target for promoting vascular repair and wound healing.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using TYMP antibody shows cytoplasmic and extracellular localization in endothelial and stromal cells. The TYMP antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TYMP - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TYMP - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TYMP - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TYMP - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TYMP+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323656557,"sku":"FY13337","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2e89414d-01bd-4850-b482-b03fd5bd7acf.jpg?v=1782237109","url":"https:\/\/www.ebiohippo.com\/products\/tymp-antibody-thymidine-phosphorylase-pd-ecgf-bha17136239","provider":"BioHippo","version":"1.0","type":"link"}