{"product_id":"u-blot-femto-sensitive-ecl-solution-bht21800005","title":"U-Blot® Femto-Sensitive ECL Solution","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eECL Western Blot Substrate\u003c\/span\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eU-Blot® Femto-Sensitive ECL Solution is a highly sensitive, non-radioactive chemiluminescent substrate based on Luminol for detecting horseradish peroxidase (HRP) immobilized on membranes during immunoblotting, with the ability to detect antigens at low femtogram antigens (ultra-sensitive).\u003c\/p\u003e\n\u003cp\u003eThe continuous luminescence lasts up to 8 hours and can be repeatedly exposed to chemiluminescent imaging systems for optimal results, as well as used for stripping immunodetection reagents and reprobing. Its high sensitivity allows greater antibody dilutions (Primary 1:5000-1:100,000; Secondary 1:100,000-1:500,000 (1 mg\/ml stock)), significantly reducing antibody consumption.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eSubstrate Type\u003c\/th\u003e\n\u003ctd\u003eLuminol-based chemiluminescent (non-radioactive)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Enzyme\u003c\/th\u003e\n\u003ctd\u003eHorseradish peroxidase (HRP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity\u003c\/th\u003e\n\u003ctd\u003eLow femtogram antigens (ultra-sensitive)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSignal Duration\u003c\/th\u003e\n\u003ctd\u003e8 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eChemiluminescent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eWestern Blot, Northern Blot, Southern Blot, Dot Blot\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecommended Antibody Dilution\u003c\/th\u003e\n\u003ctd\u003ePrimary 1:5000-1:100,000; Secondary 1:100,000-1:500,000 (1 mg\/ml stock)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eRoom temperature (2-30°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eRoom temperature (2-30°C), dry\/sealed\/dark: shelf life up to 1 year; refrigerated at 2-8°C away from light: up to 2 years.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eUsed to detect HRP-labeled probes or antibodies in Western Blot, Northern Blot, Southern Blot, Dot Blot.\u003c\/p\u003e\n\u003ch2\u003eCompatibility \u0026amp; Usage Notes\u003c\/h2\u003e\n\u003cp\u003eMix the two components in equal volumes (1:1) immediately before use and warm to room temperature. Keep the membrane wet, incubate 3-5 minutes, and avoid excess HRP, which can quench signal.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only. Not for use in diagnostic procedures. Sodium azide inhibits HRP; do not use it as a preservative in buffers. Store away from light.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"UcallM Biosciences Inc","offers":[{"title":"100 ml","offer_id":53453128171885,"sku":"W2502","price":258.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/femto-sensitive-ecl-solution.png?v=1781631772","url":"https:\/\/www.ebiohippo.com\/products\/u-blot-femto-sensitive-ecl-solution-bht21800005","provider":"BioHippo","version":"1.0","type":"link"}