{"product_id":"u-blot-nc-transfer-membrane-bhu21800001","title":"U-Blot® NC Transfer Membrane","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eTransfer Membranes\u003c\/span\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe U-Blot® NC Transfer Membrane is a nitrocellulose microporous membrane designed for protein transfer from various gel matrices. Compared to PVDF membranes, NC membranes are hydrophilic and require no prior hydrophilic treatment. It is available in two pore-size variants (0.45 µm and 0.2 µm), enabling binding of proteins across a wide molecular-weight range.\u003c\/p\u003e\n\u003cp\u003eThe 0.45 µm pore size is suitable for most proteins and offers high detection sensitivity, while the 0.2 µm pore size is ideal for proteins with a molecular weight below 20,000, enabling superior adsorption and retention of small-molecule proteins. The membrane exhibits excellent protein retention, high physical strength, and broad chemical compatibility, making it well-suited for immunological assays.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eMaterial\u003c\/th\u003e\n\u003ctd\u003eNitrocellulose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMembrane Property\u003c\/th\u003e\n\u003ctd\u003eHydrophilic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePore Sizes\u003c\/th\u003e\n\u003ctd\u003e0.2 µm and 0.45 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein Binding Capacity (BSA)\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;160 µg\/cm² (0.2 µm); \u0026gt;140 µg\/cm² (0.45 µm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat \/ Dimensions\u003c\/th\u003e\n\u003ctd\u003e30 cm x 3 m\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Methods\u003c\/th\u003e\n\u003ctd\u003eChemiluminescent, Colorimetric, Radioactive\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eWestern Blot, Northern Blot, Southern Blot\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eSuitable for Western blot, Northern blot, and Southern blot workflows, with compatibility across chemiluminescent, colorimetric, and radioactive detection systems.\u003c\/p\u003e\n\u003ch2\u003eCompatibility \u0026amp; Usage Notes\u003c\/h2\u003e\n\u003cp\u003eSelect the 0.45 µm pore size for most proteins and high sensitivity; choose the 0.2 µm pore size for proteins below ~20 kDa where superior retention of small proteins is needed.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only. Not for use in diagnostic procedures. Always wear gloves and use blunt\/flat-tipped forceps to prevent fingerprint contamination and mechanical damage. Do not fold or scratch the membrane surface, and keep it wet during the immunodetection workflow.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"UcallM Biosciences Inc","offers":[{"title":"0.2 um (\u003e160 ug\/cm2 BSA) \/ 30cm x 3m","offer_id":53453096911213,"sku":"W3N23","price":368.0,"currency_code":"USD","in_stock":true},{"title":"0.45 um (\u003e140 ug\/cm2 BSA) \/ 30cm x 3m","offer_id":53453096976749,"sku":"W3N43","price":368.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/u-blot-nc-transfer-membrane-bhu21800001.png?v=1781636564","url":"https:\/\/www.ebiohippo.com\/products\/u-blot-nc-transfer-membrane-bhu21800001","provider":"BioHippo","version":"1.0","type":"link"}