{"product_id":"u-blot-phospho-myosin-light-chain-2-ser19-mouse-mab-bha21821365","title":"U-Blot® Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003ePost-Translational Modification Antibody\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eU-Blot® Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb is a recombinant monoclonal antibody raised in Mouse that specifically recognizes \u003cstrong\u003ePhospho-Myosin Light Chain 2 (Ser19)\u003c\/strong\u003e (Phosphorylation [Ser19]). It is designed for detection and analysis of the Phosphorylation [Ser19] post-translational modification across research applications.\u003c\/p\u003e\n\u003cp\u003eMyosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+\/calmodulin-dependent manner. This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction. ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers. Phosphorylation of smooth muscle MLC2 at Ser1\/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity. Phosphorylation by cdc2 controls the timing of cytokinesis.\u003c\/p\u003e\n\u003ch2\u003eValidated Applications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003ctd\u003eWestern Blot, Immunofluorescence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecommended Dilution\u003c\/th\u003e\n\u003ctd\u003eWB 1:1000, ICC\/IF 1:50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost\u003c\/th\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003eRecombinant Monoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG1\/Kappa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactivity\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugate\u003c\/th\u003e\n\u003ctd\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification\u003c\/th\u003e\n\u003ctd\u003eProtein A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd\u003eCalculated: 20 kDa | Observed: 18 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eImmunogen \/ Antigen\u003c\/h2\u003e\n\u003cp\u003epolypeptide\u003c\/p\u003e\n\u003ch2\u003eReactivity Notes\u003c\/h2\u003e\n\u003cp\u003eThis antibody shows reactivity with Human. It targets the Phosphorylation [Ser19] modification and is suitable for Western Blot, Immunofluorescence.\u003c\/p\u003e\n\u003cdiv class=\"bhp-qc-badge\"\u003eValidated for Western Blot\u003c\/div\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e Not for diagnostic or therapeutic use. Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt \/ reconstitution.\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eExplore other UcallM Biosciences post-translational modification antibodies available through BioHippo.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UcallM Biosciences Inc","offers":[{"title":"100 uL","offer_id":53438914003309,"sku":"WT9081-100UL","price":288.0,"currency_code":"USD","in_stock":true},{"title":"300 uL","offer_id":53438914036077,"sku":"WT9081-300UL","price":368.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Ucallm_WT9081-100.jpg?v=1781281837","url":"https:\/\/www.ebiohippo.com\/products\/u-blot-phospho-myosin-light-chain-2-ser19-mouse-mab-bha21821365","provider":"BioHippo","version":"1.0","type":"link"}