{"product_id":"u-blot-wwox-phospho-tyr33-rabbit-polyclonal-antibody-bha21821121","title":"U-Blot® WWOX (Phospho Tyr33) Rabbit Polyclonal Antibody","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eAntibody\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eU-Blot® WWOX (Phospho Tyr33) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against WWOX. Recognizes Phospho. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.\u003c\/p\u003e\n\u003ch2\u003eValidated Applications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eWWOX\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost\u003c\/th\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugate\u003c\/th\u003e\n\u003ctd\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactivity\u003c\/th\u003e\n\u003ctd\u003eHuman, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eWestern Blot, Immunohistochemistry, Immunofluorescence, ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification\u003c\/th\u003e\n\u003ctd\u003eAffinity Purified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-WWOX (Y33) Polyclonal Antibody detects endogenous levels of WWOX protein only when phosphorylated at Y33.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd\u003eObserved: 55 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniProt \/ Swiss-Prot\u003c\/th\u003e\n\u003ctd\u003eQ96KM3 (Human)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eRecommended dilutions:\u003c\/strong\u003e WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200\u003c\/p\u003e\n\u003ch2\u003eImmunogen \/ Antigen\u003c\/h2\u003e\n\u003cp\u003eThe antiserum was produced against synthesized peptide derived from human WWOX around the phosphorylation site of Tyr33. AA range:18-67\u003c\/p\u003e\n\u003ch2\u003eReactivity Notes\u003c\/h2\u003e\n\u003cp\u003eConfirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003edisease:Defects in WWOX may be involved in esophageal squamous cell carcinoma (ESCC) [MIM:133239].,disease:Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.,domain:The WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.,function:Probable oxidoreductase, which acts as a tumor suppressor and plays a role in apoptosis. May function synergistically with TP53\/p53 to control genotoxic stress-induced cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death.,PTM:Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity.,similarity:Belongs to the short-chain dehydrogenases\/reductases (SDR) family.,similarity:Contains 2 WW domains.,subcellular location:Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Isoform 5 and isoform 6 may localize in the nucleus.,subunit:Interacts with TP53, TP73\/p73 and MAPK8. Interacts with MAPT\/TAU (By similarity). Forms a ternary complex with TP53 and MDM2. Interacts with ERBB4, LITAF and WBP1. May interact with COTE1\/C1orf2 and SCOTIN.,tissue specificity:Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C\/1 year(Do not lower than -25°C)\u003c\/div\u003e\n\u003c\/div\u003e","brand":"UcallM Biosciences Inc","offers":[{"title":"50 uL","offer_id":53433379979629,"sku":"WP9843-50","price":288.0,"currency_code":"USD","in_stock":true},{"title":"100 uL","offer_id":53434111164781,"sku":"WP9843-100","price":368.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Ucallm_WP9843-50.jpg?v=1781189604","url":"https:\/\/www.ebiohippo.com\/products\/u-blot-wwox-phospho-tyr33-rabbit-polyclonal-antibody-bha21821121","provider":"BioHippo","version":"1.0","type":"link"}