{"product_id":"u2os-crispr-nup96-snap-cell-bhc11100887","title":"U2OS-CRISPR-NUP96-SNAP cell","description":"The U-2 OS-CRISPR-NUP96-SNAP is a genetically modified osteosarcoma cell line derived from the parent U-2 OS human cell line. This cell line has been engineered through CRISPR\/Cas9-mediated genome editing to incorporate a SNAP-tag at the NUP96 gene, enabling the visualization and study of nuclear pore complex dynamics. Nuclear pore complexes (NPCs) are crucial for the regulation of nucleocytoplasmic transport, and NUP96 is a significant component of the NPC, playing a pivotal role in its structural integrity and function.\n\nIn U-2 OS-CRISPR-NUP96-SNAP clone no.33, the integration of the SNAP-tag at the NUP96 locus allows for specific and covalent attachment of fluorescent substrates or other chemical probes that can be used for live-cell imaging and other biochemical assays. This feature makes it an invaluable tool for investigating the molecular dynamics of nucleocytoplasmic transport, understanding NPC-related pathologies, and screening for therapeutic compounds that affect NPC function. The cell line also retains the characteristics of the parental U-2 OS line, which includes a high level of genetic stability and ease of culture, making it suitable for high-throughput screening and extended studies in cell biology.\n\nDue to the specificity of the modification at the NUP96 gene, U-2 OS-CRISPR-NUP96-SNAP clone no.33 provides a unique model for the detailed study of NPC components in the context of cellular function and dysfunction. Researchers can exploit the SNAP-tag system to selectively and rapidly label NUP96, facilitating real-time visualization of NPC dynamics under physiological and pathological conditions. This specific clone can serve as a robust platform for both basic research and applied biomedical studies, contributing significantly to the fields of cellular biology, genetics, and oncology.\n\u003cp style=\"display:none\"\u003eSKU:BHC11100887\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950227321197,"sku":"300444","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/U-2_20OS-CRISPR-NUP96-SNAP_20clone_20no.33_20_285_29_1920x1920_5f204f74-3327-46e0-a21f-37fa8d55c848.jpg?v=1769069264","url":"https:\/\/www.ebiohippo.com\/products\/u2os-crispr-nup96-snap-cell-bhc11100887","provider":"BioHippo","version":"1.0","type":"link"}