{"product_id":"ube2f-antibody-nedd8-conjugating-enzyme-e2-f-bha17135985","title":"UBE2F Antibody \/ NEDD8-conjugating enzyme E2 F","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eUBE2F Antibody \/ NEDD8-conjugating enzyme E2 F is a anti-UBE2F Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e UBE2F\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eUBE2F antibody detects NEDD8-conjugating enzyme E2 F, an E2 ubiquitin-like conjugating enzyme that catalyzes neddylation of cullin proteins, regulating cullin-RING ligase (CRL) activity. The UniProt recommended name is NEDD8-conjugating enzyme E2 F (UBE2F). This enzyme cooperates with the NEDD8-activating enzyme (NAE1\/UBA3) and specific E3 ligases to attach NEDD8 to target substrates, modulating protein degradation pathways.\u003cbr\u003e\u003cbr\u003eFunctionally, UBE2F antibody identifies a 185-amino-acid cytoplasmic enzyme that activates and transfers NEDD8 to cullin 5 and other CRL components. UBE2F works specifically with the E3 ligase RBX2 (RNF7), forming a selective neddylation module distinct from UBE2M (UBC12). Through this modification, UBE2F regulates CRL activity, affecting cell cycle progression, signal transduction, and stress responses.\u003cbr\u003e\u003cbr\u003eThe UBE2F gene is located on chromosome 5q31.2 and is expressed in multiple tissues, including testis, brain, and lung. Its expression is cell cycle-dependent and induced under oxidative stress. UBE2F ensures timely activation of CRLs, thereby influencing ubiquitin-mediated degradation of regulatory proteins such as p27 and HIF1A.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of UBE2F contributes to cancer and oxidative stress-related disorders. Overexpression promotes degradation of tumor suppressors, while inhibition can stabilize proteins that restrain cell proliferation. UBE2F has emerged as a therapeutic target for modulating proteostasis and redox balance in malignant cells.\u003cbr\u003e\u003cbr\u003eUBE2F antibody is suitable for western blotting, immunoprecipitation, and immunofluorescence to detect neddylation components and study ubiquitin-like modification systems. \u003cbr\u003e\u003cbr\u003eStructurally, UBE2F contains a conserved catalytic cysteine residue within its E2 core domain, forming a thioester bond with activated NEDD8. This structure enables transfer to substrate-bound E3 ligases. This antibody helps characterize UBE2F's mechanistic role in the neddylation cascade and its impact on CRL activity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=UBE2F - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=UBE2F - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=UBE2F - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/UBE2F - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=UBE2F+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047311171949,"sku":"FY13083","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d6e309a2-e8e2-4802-a79e-700808c0ae05.jpg?v=1782237080","url":"https:\/\/www.ebiohippo.com\/products\/ube2f-antibody-nedd8-conjugating-enzyme-e2-f-bha17135985","provider":"BioHippo","version":"1.0","type":"link"}