{"product_id":"ube2t-antibody-hspc150-bha17136078","title":"UBE2T Antibody \/ HSPC150","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eUBE2T Antibody \/ HSPC150 is a anti-UBE2T Rabbit antibody Recombinant Rabbit Monoclonal clone 31U48 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e UBE2T\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31U48, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eUBE2T antibody detects Ubiquitin-conjugating enzyme E2 T, encoded by the UBE2T gene. Ubiquitin-conjugating enzyme E2 T is a member of the E2 family of enzymes that work with E1 activating enzymes and E3 ligases to attach ubiquitin to target proteins. This modification can regulate protein degradation, localization, or activity. UBE2T is best known for its role in the Fanconi anemia DNA repair pathway, where it is required for monoubiquitination of FANCD2 and FANCI. UBE2T antibody provides researchers with a specific reagent to study DNA repair, genome stability, and ubiquitin signaling.\u003cbr\u003e\u003cbr\u003eUbiquitin-conjugating enzyme E2 T functions by transferring activated ubiquitin from the E1 enzyme to E3 ligases, which then attach ubiquitin to substrates. Research using UBE2T antibody has shown that this enzyme is critical for DNA crosslink repair. Monoubiquitination of FANCD2-FANCI is a central step in activating the Fanconi anemia pathway, allowing recruitment of downstream repair proteins to stalled replication forks. Without UBE2T activity, this repair process fails, resulting in genomic instability and increased sensitivity to DNA crosslinking agents.\u003cbr\u003e\u003cbr\u003eMutations in UBE2T cause a subtype of Fanconi anemia, a genetic disorder characterized by bone marrow failure, developmental abnormalities, and cancer predisposition. Studies with UBE2T antibody have revealed that patient-derived mutations impair the enzyme's ability to transfer ubiquitin, leading to defective DNA repair. Because of this essential function, UBE2T has emerged as a critical component of genome maintenance.\u003cbr\u003e\u003cbr\u003eBeyond inherited disease, UBE2T is implicated in cancer. Research using UBE2T antibody has demonstrated that the protein is frequently overexpressed in tumors such as breast, lung, and prostate cancers. Elevated levels enhance DNA repair capacity and promote resistance to chemotherapy. UBE2T overexpression also correlates with tumor aggressiveness, making it a potential prognostic biomarker and therapeutic target. Inhibiting UBE2T could sensitize cancer cells to DNA damaging therapies.\u003cbr\u003e\u003cbr\u003eUBE2T antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein levels across tissues, immunohistochemistry reveals expression patterns in tumors, and immunofluorescence localizes UBE2T in nuclei of proliferative cells. These applications make UBE2T antibody valuable for research into DNA repair and cancer biology.\u003cbr\u003e\u003cbr\u003eBy providing validated UBE2T antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=UBE2T - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=UBE2T - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=UBE2T - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/UBE2T - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=UBE2T+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047317791085,"sku":"FY13176","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ec618048-81f8-45eb-a63f-5e72f3729f99.jpg?v=1772019403","url":"https:\/\/www.ebiohippo.com\/products\/ube2t-antibody-hspc150-bha17136078","provider":"BioHippo","version":"1.0","type":"link"}