{"product_id":"ubtf-antibody-ubf1-upstream-binding-factor-1-bha17136233","title":"UBTF Antibody \/ UBF1 \/ Upstream binding factor 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eUBTF Antibody \/ UBF1 \/ Upstream binding factor 1 is a anti-UBTF Rabbit antibody Recombinant Rabbit Monoclonal clone 32U20 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e UBTF\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32U20, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eUBTF antibody detects Upstream binding factor 1, encoded by the UBTF gene. Upstream binding factor 1 is a nucleolar transcription factor essential for ribosomal RNA synthesis and ribosome biogenesis. UBTF antibody provides researchers with a highly specific reagent for investigating transcriptional regulation, nucleolar organization, and diseases associated with altered ribosome production.\u003cbr\u003e\u003cbr\u003eUpstream binding factor 1 binds ribosomal DNA promoter sequences and induces conformational changes that promote recruitment of RNA polymerase I and associated factors. Research using UBTF antibody has shown that the protein cooperates with selectivity factor 1 and other nucleolar proteins to form the transcription initiation complex. By bending ribosomal DNA and stabilizing transcription machinery, Upstream binding factor 1 ensures efficient expression of ribosomal RNA, a prerequisite for ribosome assembly and cellular protein synthesis.\u003cbr\u003e\u003cbr\u003eStudies with UBTF antibody have demonstrated that the protein is subject to regulation by post-translational modifications. Phosphorylation by kinases such as ERK and mTOR influences DNA binding activity and transcriptional output. This regulation allows cells to couple ribosome biogenesis with growth signals, ensuring that protein production capacity matches proliferative demands. Such control is critical during development, stress adaptation, and oncogenic transformation.\u003cbr\u003e\u003cbr\u003eIn addition to transcriptional regulation, Upstream binding factor 1 contributes to nucleolar structure. Research using UBTF antibody has revealed that UBTF localizes to transcriptionally active nucleolar regions, forming foci that align with ribosomal DNA arrays. Loss of UBTF disrupts nucleolar organization, reduces ribosomal RNA transcription, and impairs ribosome assembly. These structural roles highlight UBTF as both a functional transcription factor and a nucleolar architectural protein.\u003cbr\u003e\u003cbr\u003eDysregulation of UBTF has been implicated in cancer and neurodegenerative disease. Studies with UBTF antibody have shown that elevated UBTF activity supports increased ribosome biogenesis in tumors, fueling uncontrolled cell proliferation. Overexpression correlates with poor prognosis in certain cancers, making UBTF a potential diagnostic marker and therapeutic target. Conversely, mutations in UBTF have been associated with pediatric neurodevelopmental syndromes and neurodegeneration, linking impaired ribosome production to neuronal dysfunction. These findings underscore the dual importance of UBTF in health and disease.\u003cbr\u003e\u003cbr\u003eUpstream binding factor 1 also interacts with chromatin remodeling factors. Research using UBTF antibody has demonstrated that UBTF recruits or cooperates with histone modifiers to establish transcriptionally competent rDNA chromatin. Through these interactions, UBTF integrates epigenetic cues with transcriptional output, providing a mechanism for long-term regulation of ribosome synthesis in response to environmental signals.\u003cbr\u003e\u003cbr\u003eUBTF antibody is widely applied in chromatin immunoprecipitation, immunofluorescence, and western blotting. Chromatin immunoprecipitation identifies UBTF binding across ribosomal DNA repeats, immunofluorescence demonstrates nucleolar localization, and western blotting confirms expression levels and post-translational modifications. These experimental applications make UBTF antibody indispensable for studying transcriptional control of ribosome biogenesis.\u003cbr\u003e\u003cbr\u003eBy supplying validated UBTF antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=UBTF - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=UBTF - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=UBTF - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/UBTF - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=UBTF+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047322280301,"sku":"FY13331","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_49b98c0f-07c1-4f16-8fba-6208e6dbfb9c.jpg?v=1772019429","url":"https:\/\/www.ebiohippo.com\/products\/ubtf-antibody-ubf1-upstream-binding-factor-1-bha17136233","provider":"BioHippo","version":"1.0","type":"link"}