{"product_id":"ucf-me-t4-polynucleotide-kinase-10-u-mu-l-bhz20800091","title":"UCF.ME™ T4 Polynucleotide Kinase (10 U\/μL)","description":"\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eT4 Polynucleotide Kinase is a 5'-hydroxyl kinase for polyribonucleotides, capable of catalyzing the transfer of the γ-phosphate group from ATP to the 5'-hydroxyl terminus of oligonucleotide chains (double-stranded or single-stranded DNA or RNA) and to 3'-monophosphate nucleotides. This reaction is reversible. T4 Polynucleotide Kinase also possesses 3'-phosphatase activity, hydrolyzing 3'-phosphate groups from the 3'-phosphate terminus of oligonucleotides, deoxy-3'-monophosphate nucleotides, and deoxy-3'-diphosphate nucleotides. In the presence of ADP, T4 PNK exhibits 5'-phosphatase activity, catalyzing the exchange of 5'-phosphate groups between 5'-P-oligo\/polyribonucleotides and ATP. It is suitable for DNA library construction and probe labeling. Compared to T4 Polynucleotide Kinase (Cat#12902ES), UCF.METMT4 Polynucleotide Kinase has lower host residue, making it suitable for applications that require stricter background control, such as pathogen detection.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003ePhosphorylation of the 5' terminus of DNA or RNA for ligation reactions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eEnd labeling of DNA or RNA for probe preparation and DNA sequencing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003ePhosphorylation of the 5' terminus of mononucleotides that already have a 3'-phosphate group to prepare pNp substrates for addition to the 3' end of DNA or RNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eLabeling of the 5' terminus of oligonucleotides that have a 3'-phosphate group.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ctable border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"102%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"21%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eComponents No.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"28%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eName\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e14503ES76\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e14503ES86\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e14503ES92\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"21%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e14503-A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"28%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eUCF.METM T4 polynucleotide Kinase (10 U\/μL)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003ca name=\"OLE_LINK4\"\u003e\u003c\/a\u003e\u003cspan lang=\"EN-US\"\u003e50 µL\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e250 µL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"21%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e14503-B\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"28%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e10×T4 PNK Buffer*\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e2×1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cspan lang=\"EN-US\"\u003e \u003c\/span\u003e\u003c\/h3\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eShipping and Storage\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eThis product should be stored at -\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e25 ~ -15\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e℃\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e for 1 year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eFigures\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cdiv\u003e\u003cimg alt=\"Figure1. Monomer Phosphorylation Test\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_e7312996-01d1-43cb-bd4c-f68e88f09cad_1024x1024.png?v=1749179337\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan lang=\"EN-US\"\u003eFigure1. \u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003eMonomer Phosphorylation Test\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eNote: A 300 bp monomer ligation test was performed using different batches of UCF.METM T4 Polynucleotide Kinase (10 U\/μL) at 5 and 10 U, compared with Brand A. The gel image results showed that Yeasen's product outperformed Brand A in phosphorylation.\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"500 U","offer_id":53294149009773,"sku":"14503ES76","price":100.0,"currency_code":"USD","in_stock":true},{"title":"2500 U","offer_id":53294158086509,"sku":"14503ES86","price":260.0,"currency_code":"USD","in_stock":true},{"title":"10000 U","offer_id":53294158119277,"sku":"14503ES92","price":770.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_1024x1024_923cf239-28f7-4e56-b633-b78295864602.png?v=1778101061","url":"https:\/\/www.ebiohippo.com\/products\/ucf-me-t4-polynucleotide-kinase-10-u-mu-l-bhz20800091","provider":"BioHippo","version":"1.0","type":"link"}